Integration and validation of the ex vivo human placenta perfusion model

Journal of Pharmacological and Toxicological Methods
Sigrid ConingsKristel Van Calsteren

Abstract

The ex vivo human placenta perfusion model is an effective and non-invasive method to study transplacental passage of drugs and environmental compounds in humans. Due to many challenges and its high complexity it remains difficult to incorporate it routinely into laboratories. This article describes a step-by-step protocol for the implementation and validation of a closed-closed ex vivo perfusion model. Antipyrine, a small molecule that passes the placental barrier by passive diffusion, was used as a measurement of overlap between foetal and maternal circulation. The pressure and the flow rate in the foetal circulation, glucose consumption and pH were implemented to ensure the integrity, viability and functionality of the method. In total 89 placenta were collected of which 34 placentas were successfully perfused with antipyrine and fulfilled all quality control measurements. A foetal/maternal antipyrine concentration ratio of 0.75 was reached within 89±21 min, while 210 min were required to achieve equilibrium. The foetal pressure remained under 70 mmHg during the entire experiment. The end foetal flow was 98% of the foetal starting flow. The average glucose consumption was 0.30±0.15 μmol/min/g. Every 30 min the maternal pH de...Continue Reading

Citations

Sep 16, 2018·Annals of Biomedical Engineering·Navein ArumugasaamyJohn P Fisher
Aug 31, 2019·Cell Biology International·Thádia Evelyn de AraújoIdessania Nazareth da Costa
Sep 6, 2019·Interface Focus·Rajeendra L PemathilakaNicole N Hashemi
Jun 5, 2020·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Heqin YangHaijun Gao
Aug 28, 2020·Frontiers in Microbiology·Anja Saso, Beate Kampmann
Sep 22, 2020·Advanced Drug Delivery Reviews·Christian G Figueroa-EspadaRachel S Riley
Apr 9, 2021·Advanced Drug Delivery Reviews·Rachel E Young, Dan Dongeun Huh
Aug 30, 2021·Advanced Drug Delivery Reviews·Anjali SharmaRangaramanujam M Kannan

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