Integration of 3D Printed and Micropatterned Polycaprolactone Scaffolds for Guidance of Oriented Collagenous Tissue Formation In Vivo

Advanced Healthcare Materials
Sophia P PilipchukWilliam V Giannobile

Abstract

Scaffold design incorporating multiscale cues for clinically relevant, aligned tissue regeneration has potential to improve structural and functional integrity of multitissue interfaces. The objective of this preclinical study is to develop poly(ε-caprolactone) (PCL) scaffolds with mesoscale and microscale architectural cues specific to human ligament progenitor cells and assess their ability to form aligned bone-ligament-cementum complexes in vivo. PCL scaffolds are designed to integrate a 3D printed bone region with a micropatterned PCL thin film consisting of grooved pillars. The patterned film region is seeded with human ligament cells, fibroblasts transduced with bone morphogenetic protein-7 genes seeded within the bone region, and a tooth dentin segment positioned on the ligament region prior to subcutaneous implantation into a murine model. Results indicate increased tissue alignment in vivo using micropatterned PCL films, compared to random-porous PCL. At week 6, 30 μm groove depth significantly enhances oriented collagen fiber thickness, overall cell alignment, and nuclear elongation relative to 10 μm groove depth. This study demonstrates for the first time that scaffolds with combined hierarchical mesoscale and micros...Continue Reading

References

Apr 23, 1999·Biomaterials·R G FlemmingP F Nealey
Dec 18, 2002·Journal of Biomechanics·James H-C WangSavio L-Y Woo
Apr 2, 2003·Journal of Periodontology·Q M JinW V Giannobile
Apr 15, 2003·Journal of Cell Science·Ana I TeixeiraPaul F Nealey
Nov 19, 2005·Science·Molly M Stevens, Julian H George
Sep 5, 2006·Expert Opinion on Drug Delivery·Darnell KaiglerWilliam V Giannobile
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Elliot E Hui, Sangeeta N Bhatia
Jun 6, 2009·Angewandte Chemie·Christopher J BettingerJeffrey T Borenstein
Jun 6, 2009·Journal of Dental Research·S A Hacking, A Khademhosseini
Mar 6, 2010·Nature Protocols·Dong QinGeorge M Whitesides
May 18, 2010·Biomaterials·Chan Ho ParkWilliam V Giannobile
May 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stefan HoehmeDirk Drasdo
Jul 20, 2010·Biomaterials·Hug AubinAli Khademhosseini
Jan 5, 2011·PloS One·Jamie L IfkovitsJason A Burdick
Feb 3, 2011·Journal of Periodontology·Hector F RiosWilliam V Giannobile
Sep 22, 2011·Journal of Biomedical Materials Research. Part a·Laura KoepsellYing Deng
Oct 14, 2011·Biomaterials·Chan Ho ParkWilliam V Giannobile
Nov 19, 2011·Journal of Clinical Periodontology·Sahng G KimHyun-Duck Nah
Feb 16, 2012·Nature Communications·Marie VersaevelSylvain Gabriele
Apr 24, 2012·Biomaterials·Mehdi NikkhahAli Khademhosseini
Sep 1, 2012·Journal of Dental Research·P I EkeR J Genco
May 24, 2013·The New England Journal of Medicine·David A ZopfGlenn E Green
Aug 1, 2013·Biomaterials·Alexandre LeclercAndrew E Pelling
Nov 6, 2013·Tissue Engineering. Part C, Methods·Chan Ho ParkWilliam V Giannobile
Dec 4, 2013·Tissue Engineering. Part a·Chang H LeeJeremy J Mao
Dec 7, 2013·Journal of Clinical Periodontology·Pedro F CostaDietmar W Hutmacher
Aug 21, 2014·Journal of Dental Research·S IvanovskiP M Bartold
Oct 27, 2014·Nature Materials·Ulrike G K WegstRobert O Ritchie
Feb 24, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Sophia P PilipchukWilliam V Giannobile
Jul 1, 2015·Journal of Dental Research·G RasperiniW V Giannobile

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Citations

Sep 9, 2017·International Journal of Molecular Sciences·Chan Ho ParkYang-Jo Seol
Dec 22, 2017·Tissue Engineering. Part a·Daniel W WeisgerberBrendan A C Harley
Nov 17, 2017·Journal of the Korean Association of Oral and Maxillofacial Surgeons·Laura GaviriaJoo L Ong
Aug 28, 2018·Advanced Healthcare Materials·Cedryck VaquetteSaso Ivanovski
May 11, 2019·Orthodontics & Craniofacial Research·Ning YuWilliam V Giannobile
Sep 8, 2019·International Journal of Molecular Sciences·Chan Ho Park
Jul 7, 2018·Beilstein Journal of Nanotechnology·Reika MakitaTsutomu Sugaya
Jul 19, 2020·Journal of Clinical Medicine·Dobrila NesicIrena Sailer
Oct 20, 2018·Advanced Healthcare Materials·Sophia P PilipchukWilliam V Giannobile
Apr 12, 2019·Journal of Dental Research·C VaquetteS Ivanovski
Dec 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shan-Hui HsuCheng-Wei Chen
Dec 14, 2018·Frontiers in Bioengineering and Biotechnology·Gunpreet OberoiHermann Agis
Mar 7, 2020·Journal of Periodontal Research·Reuben J StaplesCedryck Vaquette
Aug 24, 2018·Frontiers in Physiology·Giovanna OrsiniThimios A Mitsiadis
Dec 12, 2020·International Journal of Molecular Sciences·Min Guk Kim, Chan Ho Park
Nov 19, 2020·Polymers·Obinna OkolieJames Njuguna
Dec 17, 2020·Journal of Clinical Medicine·Simon Raveau, Fabienne Jordana
Aug 1, 2020·Acta Biomaterialia·Aline LueckgenAmaia Cipitria
Apr 6, 2021·Bioactive Materials·Hyun Nyun WooChang H Lee
Jul 25, 2021·International Journal of Molecular Sciences·Hsu-Hsiang LinPo-Chun Chang
Dec 11, 2017·ACS Biomaterials Science & Engineering·HaiMing ZhaoYong He
Aug 24, 2021·Frontiers in Bioengineering and Biotechnology·Jessica M LatimerWilliam V Giannobile

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