Integration of electron microscopy and solid-state NMR analysis for new views and compositional parameters of Aspergillus fumigatus biofilms

Medical Mycology
Courtney ReichhardtLynette Cegelski

Abstract

The general ability and tendency of bacteria and fungi to assemble into bacterial communities, termed biofilms, poses unique challenges to the treatment of human infections. Fungal biofilms, in particular, are associated with enhanced virulence in vivo and decreased sensitivity to antifungals. Much attention has been given to the complex cell wall structures in fungal organisms, yet beyond the cell surface, Aspergillus fumigatus and other fungi assemble a self-secreted extracellular matrix that is the hallmark of the biofilm lifestyle, protecting and changing the environment of resident members. Elucidation of the chemical and molecular detail of the extracellular matrix is crucial to understanding how its structure contributes to persistence and antifungal resistance in the host. We present a summary of integrated analyses of A. fumigatus biofilm architecture, including hyphae and the extracellular matrix, by scanning electron microscopy and A. fumigatus matrix composition by new top-down solid-state NMR approaches coupled with biochemical analysis. This combined methodology will be invaluable in formulating quantitative and chemical comparisons of A. fumigatus isolates that differ in virulence and are more or less resistant t...Continue Reading

References

Aug 15, 1986·Archives of Biochemistry and Biophysics·T FukamizoG S Jacob
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·K Lewis
Apr 28, 2001·International Journal of Biological Macromolecules·M L DuarteJ Rocha
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Jan 16, 2003·Trends in Microbiology·L Julia Douglas
Aug 26, 2004·Antimicrobial Agents and Chemotherapy·Mohammed A Al-Fattani, L Julia Douglas
Nov 10, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Pranab K Mukherjee, Jyotsna Chandra
Jul 20, 2006·Journal of Medical Microbiology·Mohammed A Al-Fattani, L Julia Douglas
Oct 24, 2006·Current Opinion in Microbiology·Jill R Blankenship, Aaron P Mitchell
Dec 5, 2006·Nature Reviews. Microbiology·Kim Lewis
Mar 21, 2007·Cellular Microbiology·Anne BeauvaisJean Paul Latgé
Aug 7, 2007·Journal of Bacteriology·Hans-Curt FlemmingDaniel J Wozniak
May 21, 2008·Studies in Mycology·S A BalajeeR A Samson
Jul 26, 2008·Medical Mycology·Eilidh MowatGordon Ramage
Aug 3, 2010·Nature Reviews. Microbiology·Hans-Curt Flemming, Jost Wingender
Jan 11, 2013·Virulence·François L MayerBernhard Hube
Jul 6, 2013·Journal of Molecular Biology·Oscar A McCrateLynette Cegelski
Aug 27, 2013·Current Genetics·Lotte Mathé, Patrick Van Dijck
Sep 17, 2013·Bioorganic & Medicinal Chemistry Letters·Lynette Cegelski
Jun 10, 2014·Biochimica Et Biophysica Acta·Courtney ReichhardtLynette Cegelski
Jul 1, 2014·Molecular Physics·Courtney Reichhardt, Lynette Cegelski
Aug 7, 2014·MBio·Robert ZarnowskiDavid R Andes
Mar 24, 2015·Journal of Magnetic Resonance·Lynette Cegelski
Sep 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joseph A H Romaniuk, Lynette Cegelski
Aug 4, 2016·Future Medicinal Chemistry·Courtney ReichhardtLynette Cegelski
Jan 20, 2018·Science·Wiriya ThongsomboonLynette Cegelski
Jul 18, 2018·Nature Communications·Xue KangTuo Wang

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Citations

Jul 25, 2019·International Journal of Molecular Sciences·Yvana GlasenappJutta Papenbrock
Sep 16, 2020·Journal of Experimental Botany·Alan WankeAlga Zuccaro
Oct 31, 2020·Journal of Fungi·Claudia Rodriguez-de la NovalAllan J Guimarães

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