Integration of immunodeficiency virus in oocytes via intracytoplasmic injection: possible but extremely unlikely

Fertility and Sterility
Marjan M C SteenvoordenSjoerd Repping

Abstract

To determine if human oocytes can be infected with HIV-1 via intracytoplasmic injection and to determine the infection threshold. Twenty-eight donated immature and unfertilized human oocytes from HIV-negative women were injected with 4 × 10(4) HIV-1 virions and 13 oocytes were used as uninjected controls. To determine the infection threshold, 543 cat oocytes were injected with 4 × 10(4), 4 × 10(2), or 40 copies of feline immunodeficiency virus (FIV) and 376 oocytes were used as controls. Academic hospital. PATIENT(S)/ANIMAL(S): Donated immature human oocytes and mature cat oocytes. Injection with HIV-1 or FIV. Viral integration as measured by fluorescent in situ hybridization with HIV-1-specific probes or by nested FIV polymerase chain reaction. We detected viral integration in three of 28 (11%) human oocytes injected with 4 × 10(4) copies of HIV-1. When injected with high dose FIV (4 × 10(4) copies) 16%-49% of cat oocytes showed viral integration. This decreased to 2%-7% and 0.6%-1.8% when an intermediate (4 × 10(2) copies) or low (40 copies) dose was injected, respectively. Human and cat oocytes can be infected with HIV-1 and FIV respectively, when injected with high amounts of virus. The probability of viral integration is e...Continue Reading

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Citations

Jul 28, 2020·Virus Research·Antoinette Cornelia van der Kuyl, Ben Berkhout

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