Integration of isothermal amplification with quantum dot-based fluorescence resonance energy transfer for simultaneous detection of multiple microRNAs

Chemical Science
Juan HuChun-Yang Zhang

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that regulate important physiological processes, and their dysregulation is associated with various human diseases. Simultaneous sensitive detection of multiple miRNAs may facilitate early clinical diagnosis. In this research, we demonstrate for the first time the integration of hyperbranched rolling circle amplification (HRCA) with quantum dot (QD)-based fluorescence resonance energy transfer (FRET) for the simultaneous detection of multiple microRNAs with a single-color QD as the donor and two fluorescent dyes as the acceptors. We used miR-21 and miR-221 as target miRNAs. We designed two circular templates which may specifically hybridize with miR-21 and miR-221, respectively, for the initiation of the HRCA reaction. The products of the HRCA reaction may hybridize with both capture probes and reporter probes to form the biotinylated acceptor-labeled sandwich hybrids. The resultant sandwich hybrids can assemble on the surface of the QD, enabling efficient FRET between the QD and the acceptors, with the Cy3 signal indicating the presence of miR-21 and the Texas Red signal indicating the presence of miR-221. This assay has significant advantages of simplicity and low cost. The HRCA re...Continue Reading

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Citations

Dec 13, 2019·Chemical Communications : Chem Comm·Yanan WuZhaohui Li
Dec 14, 2018·Chemical Science·Xiangdan MengXueji Zhang
Oct 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fei MaChun-Yang Zhang
Jul 28, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Piaopiao ChenJia Geng
Nov 27, 2019·Molecular Aspects of Medicine·Guillaume GinesValerie Taly
Mar 16, 2021·Trends in Biotechnology·Shuzhen YueSai Bi
Sep 23, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Reddhy MahleRintu Banerjee
Dec 17, 2021·Advanced Science·Xiao-Long ZhangRuo Yuan

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Methods Mentioned

BETA
fluorescence imaging
FRET
electrophoresis
Fluorescence

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