Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis

Chemical Science
Min Li, Robbyn K Anand

Abstract

We present integration of selective single-cell capture at an array of wireless electrodes (bipolar electrodes, BPEs) with transfer into chambers, reagent exchange, fluidic isolation and rapid electrical lysis in a single platform, thus minimizing sample loss and manual intervention steps. The whole process is achieved simply by exchanging the solution in a single inlet reservoir and by adjusting the applied voltage at a pair of driving electrodes, thus making this approach particularly well-suited for a broad range of research and clinical applications. Further, the use of BPEs allows the array to be scalable to increase throughput. Specific innovations reported here include the incorporation of a leak channel to balance competing flow paths, the use of 'split BPEs' to create a distinct recapture and electrical lysis point within the reaction chamber, and the dual purposing of an ionic liquid as an immiscible phase to seal the chambers and as a conductive medium to permit electrical lysis at the split BPEs.

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Citations

Sep 24, 2019·Small·Xing XuChaoyong Yang
Feb 8, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Laurent BouffierNeso Sojic
Nov 6, 2020·Electrophoresis·Kel S Hakim, Blanca H Lapizco-Encinas
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Dec 8, 2020·Analytical Chemistry·Keke HuAndrew G Ewing
Nov 25, 2020·Analytical Chemistry·Kira L Rahn, Robbyn K Anand
Nov 16, 2021·ACS Omega·Andie J RobinsonFrankie J Rawson

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