Integration of miRNA and protein profiling reveals coordinated neuroadaptations in the alcohol-dependent mouse brain

PloS One
Giorgio GoriniR Dayne Mayfield

Abstract

The molecular mechanisms underlying alcohol dependence involve different neurochemical systems and are brain region-dependent. Chronic Intermittent Ethanol (CIE) procedure, combined with a Two-Bottle Choice voluntary drinking paradigm, represents one of the best available animal models for alcohol dependence and relapse drinking. MicroRNAs, master regulators of the cellular transcriptome and proteome, can regulate their targets in a cooperative, combinatorial fashion, ensuring fine tuning and control over a large number of cellular functions. We analyzed cortex and midbrain microRNA expression levels using an integrative approach to combine and relate data to previous protein profiling from the same CIE-subjected samples, and examined the significance of the data in terms of relative contribution to alcohol consumption and dependence. MicroRNA levels were significantly altered in CIE-exposed dependent mice compared with their non-dependent controls. More importantly, our integrative analysis identified modules of coexpressed microRNAs that were highly correlated with CIE effects and predicted target genes encoding differentially expressed proteins. Coexpressed CIE-relevant proteins, in turn, were often negatively correlated wit...Continue Reading

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Citations

Jun 19, 2014·Nature Reviews. Molecular Cell Biology·Michal Breker, Maya Schuldiner
Jun 25, 2015·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Dana MostR Dayne Mayfield
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Mar 4, 2017·Neuropharmacology·Anna S Warden, R Dayne Mayfield
May 23, 2018·Alcoholism, Clinical and Experimental Research·Ovidiu Dan IancuRobert Hitzemann
Aug 1, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Giorgio GoriniR Dayne Mayfield
Dec 17, 2019·Alcoholism, Clinical and Experimental Research·Nicole A R WalterRobert Hitzemann
Dec 19, 2020·Wiley Interdisciplinary Reviews. RNA·Austin M GowenSowmya V Yelamanchili
May 2, 2021·Molecular Neurobiology·Neide VieiraNuno Sousa

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Datasets Mentioned

BETA
GSE48576

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

WGCNA
R
R Models for Microarray Data ( LIMMA ) Gene Correlation Networ...
DeCyder
MASCOT
[UNK]
Ingenuity Pathway Core
qbasePLUS
Bioconductor
Imagene

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