Integration of physical and genetic maps of common bean through BAC-derived microsatellite markers.

BMC Genomics
Juana M CórdobaM W Blair

Abstract

Common bean (Phaseolus vulgaris L.) is the most important legume for direct human consumption and the goal of this study was to integrate a recently constructed physical map for the species with a microsatellite based genetic map using a BAC library from the genotype G19833 and the recombinant inbred line population DOR364 x G19833. We searched for simple sequence repeats (SSRs) in the 89,017 BAC-end sequences (BES) from the physical map and genetically mapped any polymorphic BES-SSRs onto the genetic map. Among the BES it was possible to identify 623 contig-linked SSRs, most of which were highly AT-rich. A subgroup of 230 di-nucleotide and tri-nucleotide based SSR primer pairs from these BACs was tested on the mapping parents with 176 single copy loci and 114 found to be polymorphic markers. Of these, 99 were successfully integrated into the genetic map. The 99 linkages between the genetic and physical maps corresponded to an equal number of contigs containing a total of 5,055 BAC clones. Class II microsatellites were more common in the BES than longer class I microsatellites. Both types of markers proved to be valuable for linking BAC clones to the genetic map and were successfully placed across all 11 linkage groups. The int...Continue Reading

References

May 25, 1984·Nucleic Acids Research·D Tautz, M Renz
May 8, 1999·Genome Génome / Conseil National De Recherches Canada·B HüttelG Kahl
Sep 13, 2000·Nucleic Acids Research·Q YuanR Buell
Nov 10, 2000·Chromosoma·C Schlötterer
Nov 15, 2000·Genome Research·C SoderlundL French
Dec 5, 2000·Genetics·L RamsayR Waugh
Dec 6, 2000·Genome Biology·J L Bennetzen
Mar 23, 2002·The Plant Cell·Mingsheng ChenRod A Wing
Aug 2, 2002·Molecular Cell·Bettina HarrChristian Schlötterer
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I. MétaisD. Peltier
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A PedrosaD Schweizer
Jul 18, 2003·The New England Journal of Medicine·Albert de la Chapelle
Sep 25, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M W BlairJ Tohme
Jul 22, 2004·Nature Reviews. Genetics·Blake C MeyersMichele Morgante
Jan 19, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M I Chacón SD G Debouck
Apr 6, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B YükselA H Paterson
Apr 15, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M W BlairS E Beebe
Feb 9, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jeffry L ShultzDavid A Lightfoot
May 16, 2007·Genome Génome / Conseil National De Recherches Canada·Luiz Ricardo HanaiMaria Lucia Carneiro Vieira
Mar 22, 2008·Genome Génome / Conseil National De Recherches Canada·Randy C ShoemakerScott Jackson
May 31, 2008·BMC Bioinformatics·Frank M YouOlin D Anderson
Sep 12, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Mathew W BlairDidier Peltier
Mar 31, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Andrea Pedrosa-HarandDieter Schweizer
Nov 26, 2009·Genome Génome / Conseil National De Recherches Canada·Matthew W BlairNatalia Hurtado

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Citations

Jul 26, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Andrés J CortésMatthew W Blair
Aug 15, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sylvie CloutierMitali Banik
Jul 3, 2013·Functional & Integrative Genomics·Naser PoursarebaniNils Stein
Aug 4, 2011·Journal of Biomedicine & Biotechnology·Kangfu Yu
Apr 9, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Abhishek BohraRajeev K Varshney
Mar 13, 2012·Annual Review of Plant Biology·Nevin D Young, Arvind K Bharti
Aug 29, 2014·Plant Molecular Biology·Bárbara Salomão de Faria MüllerRosana Pereira Vianello
Feb 10, 2016·Saudi Journal of Biological Sciences·Sajad Majeed ZargarArjun Sharma
May 27, 2014·Frontiers in Plant Science·Samira Mafi MoghaddamPhillip E McClean
Nov 7, 2016·Applied Biochemistry and Biotechnology·Reetika MahajanHumaira Sonah
Nov 24, 2017·International Journal of Molecular Sciences·Ana M GonzálezMarta Santalla
Oct 11, 2012·G3 : Genes - Genomes - Genetics·Parichart NinwichianZhanjiang Liu
May 1, 2012·Molecular Ecology Resources·Matthew W BlairPrem Sharma
Aug 28, 2020·Molecular Biology Reports·Nancy GuptaR K Salgotra

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Datasets Mentioned

BETA
G19833

Methods Mentioned

BETA
PCR
Fluorescence

Software Mentioned

FPC

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