Integration of RNAi and RNA-seq uncovers the immune responses of Epinephelus coioides to L321_RS19110 gene of Pseudomonas plecoglossicida

Fish & Shellfish Immunology
Beibei ZhangQingpi Yan

Abstract

Pseudomonas plecoglossicida is a threatening and important pathogen in aquaculture and causes heavy losses. Expression of L321_RS19110 was found significant up-regulated at 18 °C than at 28 °C, which was confirmed by quantitative real-time PCR. RNAi significantly reduced the content of L321_RS19110 mRNA of P. plecoglossicida, and exhibited the best efficiency of gene silencing with a reduction of 84.9%. Compared with the wild type strain, the infection of L321_RS19110-RNAi-1 strain resulted in the onset time delay, and 30% reduction in mortality of Epinephelus coioides, as well as alleviates in the symptoms of E. coioides spleen. Moreover, compared with wild type strain, the gene silence of L321_RS19110 in P. plecoglossicida resulted in a significant change in transcriptome of infected E. coioides. The results of KEGG analysis showed that genes of chemokine signaling pathway and cytokine-cytokine receptor interaction, digestion and absorption of proteins (non-specific immune) and antigen processing and presentation pathways were most affected by L321_RS19110-RNAi of P. plecoglossicida. In these immune pathways, the most affected immune genes were associated with different number of non-coding RNAs. Among them, most lncRNAs and ...Continue Reading

Citations

Apr 9, 2019·Journal of Fish Diseases·Gang LuoQingpi Yan
Nov 27, 2019·Journal of Fish Diseases·Leilei MaoQingpi Yan
Apr 10, 2021·Journal of Agricultural and Food Chemistry·Haitham G Abo-Al-Ela

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