PMID: 9193068May 1, 1997Paper

Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: evidence for multiple complex patterns of integration

The Plant Journal : for Cell and Molecular Biology
M E KononovStanton B. Gelvin

Abstract

During the process of crown gall tumorigenesis, Agrobacterium tumefaciens transfers part of the tumor-inducing (Ti) plasmid, the T-DNA, to a plant cell where it eventually becomes stably integrated into the plant genome. Directly repeated DNA sequences, called T-DNA borders, define the left and the right ends of the T-DNA. The T-DNA can be physically separated from the remainder of the Ti-plasmid, creating a 'binary vector' system; this system is frequently used to generate transgenic plants. Scientists initially thought that only those sequences located between T-DNA left and right borders transferred to the plant. More recently, however, several reports have appeared describing the integration of the non-T-DNA binary vector 'backbone' sequences into the genome of transgenic plants. In order to investigate this phenomenon, we constructed T-DNA binary vectors containing a nos-nptll gene within the T-DNA and a mas2'-gusA (beta-glucuronidase) gene outside the T-DNA borders. We regenerated kanamycin-resistant transgenic tobacco plants and analyzed these plants for the expression of the vector-localized gusA gene and for the presence of binary vector backbone sequences. Approximately one-fifth of the plants expressed detectable GUS...Continue Reading

Citations

Mar 11, 2006·Plant Cell Reports·Jose R VidalBruce I Reisch
Jun 24, 2010·Plant Cell Reports·Massimo ConfalonieriAlma Balestrazzi
Mar 4, 2005·Molecular Genetics and Genomics : MGG·Akiko NakanoYasuhiko Mukai
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Oct 2, 2009·Plant Molecular Biology·Xi CaoDe Ye
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Oct 4, 2002·Comptes rendus biologies·Dominique OrtegaMichel Delseny
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Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Stanton B Gelvin
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