Integration of the blaNDM-1 carbapenemase gene into a novel SXT/R391 integrative and conjugative element in Proteus vulgaris.

The Journal of Antimicrobial Chemotherapy
Ling-Han KongHong-Ning Wang

Abstract

To characterize the genetic environment of the carbapenem resistance determinant in Proteus vulgaris of swine origin. The carbapenem-resistant P. vulgaris strain BC22 was isolated from a faecal swab from a diseased pig with diarrhoea in Sichuan Province of China in 2018. The presence of carbapenemase genes was screened by PCR. WGS and bioinformatics analysis were performed to analyse the genetic environment of the carbapenem resistance determinant. P. vulgaris strain BC22 was found to harbour the carbapenemase gene blaNDM-1. WGS data revealed that blaNDM-1 was located in a truncated ISAba125 composite transposon. The carbapenem resistance gene blaNDM-1 and 20 other resistance genes, including the multiresistance gene cfr and the bifunctional aminoglycoside/quinolone resistance gene aac(6')-lb-cr, were located in a novel SXT/R391 integrative and conjugative element (ICE). This new SXT/R391 ICE of 148.7 kb was chromosomally located, and could be transferred to Escherichia coli. Here, we report a carbapenemase gene, blaNDM-1, integrated into an SXT/R391 ICE. Our study highlights that this SXT/R391 ICE may facilitate the dissemination of clinically important resistance genes such as blaNDM-1, cfr and aac(6')-lb-cr.

References

Mar 15, 2011·Diagnostic Microbiology and Infectious Disease·Laurent PoirelPatrice Nordmann
Jul 15, 2011·The Journal of Antimicrobial Chemotherapy·Caterina MataMark Toleman
Jul 29, 2011·The Journal of Antimicrobial Chemotherapy·Yu WangJian-Zhong Shen
Nov 15, 2011·Trends in Microbiology·Patrice NordmannDavid M Livermore
Feb 9, 2012·Antimicrobial Agents and Chemotherapy·M A TolemanT R Walsh
Oct 16, 2012·Lancet·Elizabeth DarleyTimothy Walsh
Apr 23, 2014·Antimicrobial Agents and Chemotherapy·Lim S JonesKarthikeyan K Kumarasamy
Jun 25, 2015·Microbiology Spectrum·Nicolas Carraro, Vincent Burrus
Oct 17, 2015·Annual Review of Genetics·Christopher M Johnson, Alan D Grossman
Jan 24, 2018·Antimicrobial Agents and Chemotherapy·Yan-Peng ChenHong-Ning Wang
Feb 1, 2019·Clinical Microbiology Reviews·Wenjing WuZhiyong Zong

❮ Previous
Next ❯

Citations

Apr 24, 2021·The Journal of Antimicrobial Chemotherapy·Aline I MoserAndrea Endimiani
Jun 3, 2021·Clinical Microbiology Reviews·Stefan SchwarzYang Wang
Jul 3, 2021·Frontiers in Cellular and Infection Microbiology·Xinhua LuoDongsheng Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Related Papers

Wei sheng wu xue bao = Acta microbiologica Sinica
Peng Luo, Chaoqun Hu
Antimicrobial Resistance and Infection Control
Deepjyoti PaulAtanu Chakravarty
Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Shiela ChetriAmitabha Bhattacharjee
Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
Tawaf Ali Shah, Rabaab Zahra
Antimicrobial Agents and Chemotherapy
Sally R Partridge, Jonathan R Iredell
© 2021 Meta ULC. All rights reserved