Integrative analyses of transcriptomics and metabolomics in Raphidocelis subcapitata treated with clarithromycin

Chemosphere
Jianglin PengJinxi Song

Abstract

As a macrolide antibiotic, clarithromycin (CLA) has a high detection rate in surface water and sewage treatment plant effluents worldwide, posing a considerably high ecological risk to aquatic ecosystem. However, algal transcriptome and metabolome in response to CLA remains largely unknown. In this study, a model alga Raphidocelis subcapitata (R. subcapitata), was exposed to CLA at the concentrations of 0, 3, 10, and 15 μg L-1. Transcriptomic analysis was performed for all the treatment groups, whereas metabolomics was merely applied to 0, 3, and 10 μg L-1 groups because of the limited amount of algal biomass. After 7 d cultivation, the growth of R. subcapitata was significantly hindered at the concentrations above 10 μg L-1. A total of 115, 1833, 2911 genes were differentially expressed in 3, 10, and 15 μg L-1 groups, respectively; meanwhile, 134 and 84 differentially accumulated metabolites (DAMs) were found in the 3 and 10 μg L-1 groups. Specifically, expression levels of DEGs and DAMs related to xenobiotic metabolism, electron transport and energy synthesis were dysregulated, leading to the produced reactive oxygen species (ROS). To confront the CLA-induced injury, the biosynthesis of unsaturated fatty acids and carotenoids...Continue Reading

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Citations

Jul 10, 2021·Ecotoxicology and Environmental Safety·Yufeng MaoQiang He
Jul 30, 2021·Ecotoxicology and Environmental Safety·Ana Catarina AlmeidaAdam Lillicrap
Aug 8, 2021·Environmental Science and Pollution Research International·Jia DuLi Fu

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