Integrative analysis of gut microbiota composition, host colonic gene expression and intraluminal metabolites in aging C57BL/6J mice

Aging
Benthe van der LugtWilma T Steegenga

Abstract

The aging process is associated with diminished colonic health. In this study, we applied an integrative approach to reveal potential interactions between determinants of colonic health in aging C57BL/6J mice. Analysis of gut microbiota composition revealed an enrichment of various potential pathobionts, including Desulfovibrio spp., and a decline of the health-promoting Akkermansia spp. and Lactobacillus spp. during aging. Intraluminal concentrations of various metabolites varied between ages and we found evidence for an increased gut permeability at higher age. Colonic gene expression analysis suggested that during the early phase of aging (between 6 and 12 months), expression of genes involved in epithelial-to-mesenchymal transition and (re)organization of the extracellular matrix were increased. Differential expression of these genes was strongly correlated with Bifidobacterium spp. During the later phase of aging (between 12 and 28 months), gene expression profiles pointed towards a diminished antimicrobial defense and were correlated with an uncultured Gastranaerophilales spp. This study demonstrates that aging is associated with pronounced changes in gut microbiota composition and colonic gene expression. Furthermore, th...Continue Reading

References

Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Apr 7, 2006·BMC Bioinformatics·Jason Ernst, Ziv Bar-Joseph
Apr 24, 2007·Journal of Applied Microbiology·E J Woodmansey
Jan 21, 2009·Cell Research·Jian XuRik Derynck
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Jun 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Marcus J ClaessonPaul W O'Toole
Jul 29, 2010·Physiological Reviews·Inna SekirovB Brett Finlay
Feb 25, 2011·Age·Elena BiagiPatrizia Brigidi
May 11, 2012·Gut Microbes·Paul Hofman, Valérie Vouret-Craviari
Nov 15, 2012·BioData Mining·Ignacio GonzálezSébastien Déjean
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Dec 12, 2012·Biochimica Et Biophysica Acta·Nicol HutchisonJeremy S Duffield
Dec 12, 2012·Frontiers in Physiology·Franck CarboneroH Rex Gaskins
Feb 26, 2013·Nature Reviews. Microbiology·Felix Sommer, Fredrik Bäckhed
Jun 12, 2013·Cell·Carlos López-OtínGuido Kroemer
Nov 7, 2013·MBio·Joseph P ZackularPatrick D Schloss
Dec 18, 2013·Bioinformatics·Andreas KrämerStuart Tugendreich
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Feb 26, 2014·Nature Reviews. Immunology·Lance W Peterson, David Artis
Apr 9, 2014·Genome Biology and Evolution·Rochelle M SooPhilip Hugenholtz
Jul 1, 2014·World Journal of Gastroenterology : WJG·Li ZhongMihai Covasa
Sep 23, 2014·Nature Reviews. Immunology·Allan M Mowat, William W Agace
Dec 19, 2014·Microbiome·Morgan Gi LangilleRobert G Beiko
Mar 12, 2015·Journal of Immunology Research·Marcelo José Barbosa SilvaLiliane Martins dos Santos
Feb 16, 2016·Microbial Pathogenesis·Muriel DerrienWillem M de Vos
May 18, 2016·Current Biology : CB·Elena BiagiMarco Candela
Oct 26, 2016·Mechanisms of Ageing and Development·Sulev KõksRoosmarijn E Vandenbroucke
Oct 30, 2016·Trends in Endocrinology and Metabolism : TEM·Claudio FranceschiStefano Salvioli

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Citations

Aug 29, 2019·Physiological Reviews·John F CryanTimothy G Dinan
Oct 8, 2019·Neurological Research·Jian-Jun ChenPeng Xie
Nov 30, 2018·Current Opinion in Clinical Nutrition and Metabolic Care·Andrea TicinesiAntonio Nouvenne
Oct 6, 2020·Communicative & Integrative Biology·Hugo C BarretoIsabel Gordo
Jan 5, 2021·Archives of Microbiology·Gang YaoShuguang Wu
Apr 4, 2021·Journal of Clinical Medicine·Jorge OjedaPía M Vidal

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Datasets Mentioned

BETA
SRP145060
GSE113257

Methods Mentioned

BETA
scrapings
PCA
NMR
Ubiquitination
PCR
ELISA

Software Mentioned

Cytoscape
aldex2
Canoco
MultiExperiment Viewer
GraphPad
IPA
Casava
Olympus Cell ^ B
Ingenuity Pathway Analysis ( IPA )
CellSense Entry

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