Integrative analysis of postharvest chilling injury in cherry tomato fruit reveals contrapuntal spatio-temporal responses to ripening and cold stress

Scientific Reports
Karin AlbornozDiane M Beckles

Abstract

Postharvest chilling injury (PCI) reduces fruit quality and shelf-life in tomato (Solanum lycopersicum L.). PCI has been traditionally studied in the pericarp, however its development is likely heterogeneous in different fruit tissues. To gain insight into PCI's spatio-temporal development, we used postharvest biomarkers e.g. respiration and ethylene rates, ion leakage etc., to confirm the occurrence of PCI, and compared these data with molecular (gene expression), biophysical (MRI data) and biochemical parameters (Malondialdehyde (MDA) and starch content) from the pericarp or columella. Tissues were stored at control (12.5 °C) or PCI-inducing temperatures (2.5 or 5 °C) followed by rewarming at 20 °C. MRI and ion leakage revealed that cold irreversibly impairs ripening-associated membrane liquefaction; MRI also showed that the internal and external fruit tissues responded differently to cold. MDA and especially starch contents, were affected by chilling in a tissue-specific manner. The expression of the six genes studied: ACO1 and ACS2 (ripening), CBF1 (cold response), DHN, AOX1a and LoxB (stress-related) showed non-overlapping temporal and spatially-specific responses. Overall, the data highlighted the interconnectedness of fr...Continue Reading

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Citations

Jan 2, 2021·Horticulture Research·Emma N ShipmanDiane M Beckles
Jun 29, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Salika RamazanRiffat John
Jan 5, 2022·Journal of Food Biochemistry·Francisco Delgado-VargasJosé A López-Valenzuela

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Methods Mentioned

BETA
MDA
PCA
environmental challenge
NMR
PCR

Software Mentioned

RStudio
Excel
pca3d
SigmaPlot
Metaboanalyst
SAS
R
corrplot
factoextra
Sol Genomics

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