Integrative and Conjugative Elements (ICEs) in Pasteurellaceae Species and Their Detection by Multiplex PCR

Frontiers in Microbiology
Michal BekerStephen Douthwaite

Abstract

Strains of the Pasteurellaceae bacteria Pasteurella multocida and Mannheimia haemolytica are major etiological agents of bovine respiratory disease (BRD). Treatment of BRD with antimicrobials is becoming more challenging due to the increasing occurrence of resistance in infecting strains. In Pasteurellaceae strains exhibiting resistance to multiple antimicrobials including aminoglycosides, beta-lactams, macrolides and sulfonamides, the resistance determinants are often chromosomally encoded within integrative and conjugative elements (ICEs). To gain a more comprehensive picture of ICE structures, we sequenced the genomes of six strains of P. multocida and four strains of M. haemolytica; all strains were independent isolates and eight of them were multiple-resistant. ICE sequences varied in size from 49 to 79 kb, and were comprised of an array of conserved genes within a core region and varieties of resistance genes within accessory regions. These latter regions mainly account for the variation in the overall ICE sizes. From the sequence data, we developed a multiplex PCR assay targeting four conserved core genes required for integration and maintenance of ICE structures. Application of this assay on 75 isolates of P. multocida ...Continue Reading

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Citations

Nov 5, 2021·Bioinformatics·Alexander G McFarlandErica M Hartmann

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Datasets Mentioned

BETA
CP003022.1
CP005383.1

Methods Mentioned

BETA
ICE
ICEs
PCR
nuclear magnetic resonance
GAM

Software Mentioned

SSPACE premium scaffolder
Artemis Comparison Tool ACT
GeneRuler
Mauve
Artemis sequence visualization
CMG Comparative Microbial Genomic software
RAST
Island viewer

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