Integrative omics to detect bacteremia in patients with febrile neutropenia

PloS One
Rachel S KellyDaniel J Pallin

Abstract

Cancer chemotherapy-associated febrile neutropenia (FN) is a common condition that is deadly when bacteremia is present. Detection of bacteremia depends on culture, which takes days, and no accurate predictive tools applicable to the initial evaluation are available. We utilized metabolomics and transcriptomics to develop multivariable predictors of bacteremia among FN patients. We classified emergency department patients with FN and no apparent infection at presentation as bacteremic (cases) or not (controls), according to blood culture results. We assessed relative metabolite abundance in plasma, and relative expression of 2,560 immunology and cancer-related genes in whole blood. We used logistic regression to identify multivariable predictors of bacteremia, and report test characteristics of the derived predictors. For metabolomics, 14 bacteremic cases and 25 non-bacteremic controls were available for analysis; for transcriptomics we had 7 and 22 respectively. A 5-predictor metabolomic model had an area under the receiver operating characteristic curve of 0.991 (95%CI: 0.972,1.000), 100% sensitivity, and 96% specificity for identifying bacteremia. Pregnenolone steroids were more abundant in cases and carnitine metabolites we...Continue Reading

References

Jul 1, 1985·JPEN. Journal of Parenteral and Enteral Nutrition·G NanniM Castagneto
May 3, 2001·The American Journal of Clinical Nutrition·W DrumlG Kleinberger
Aug 9, 2003·Nature Reviews. Drug Discovery·Jeremy K Nicholson, Ian D Wilson
Dec 14, 2004·Annals of the New York Academy of Sciences·Giuseppe FamularoLuciana Mosca
Feb 20, 2008·PLoS Pathogens·Shalaka SamantAlexander A Neyfakh
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew N HotsonGarry P Nolan
Apr 8, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei ShaSteven H Zeisel
Apr 27, 2010·Human Molecular Genetics·Jiyoung AhnDemetrius Albanes
Aug 25, 2010·Critical Care : the Official Journal of the Critical Care Forum·Katsunori YanagiharaMasaki Kitajima
Jan 6, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alison G Freifeld Infectious Diseases Society of Americaa
Jan 25, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alison G Freifeld Infectious Diseases Society of America
Mar 2, 2011·PloS One·Nikolaos PsychogiosDavid S Wishart
Nov 24, 2011·Clinical Chemistry·Eugene P Rhee, Robert E Gerszten
Apr 27, 2012·Bioanalysis·Tiziana PacchiarottaOleg A Mayboroda
Jan 16, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Christopher R FlowersScott D Ramsey
Jul 5, 2013·Journal of Oncology Pharmacy Practice : Official Publication of the International Society of Oncology Pharmacy Practitioners·Derek WeyckerGary H Lyman
Jun 10, 2014·Clinical Oncology : a Journal of the Royal College of Radiologists·E DayL Hughes-Davies
Aug 26, 2014·Nature Biotechnology·Davide RissoSandrine Dudoit
Jan 13, 2015·Medical Microbiology and Immunology·Evgeny A IdelevichUNKNOWN Molecular Diagnostics of Sepsis Study Group
May 20, 2015·Biomarkers in Medicine·Ilana C van Rensburg, André G Loxton
Jun 19, 2015·PLoS Genetics·Jörg BartelFabian J Theis
Jul 29, 2015·Intensive Care Medicine Experimental·Marie-Angélique CazalisGuillaume Monneret
Aug 16, 2015·Infection·Martin E RichterMichael Kiehntopf
Aug 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Lining GuoC Thomas Caskey
Sep 17, 2015·Current Opinion in Infectious Diseases·Karin A Thursky, Leon J Worth
Feb 2, 2016·Advances in Protein Chemistry and Structural Biology·Nadia KoenDu Toit Loots
May 26, 2016·Diagnostic Microbiology and Infectious Disease·Yvonne ReersUNKNOWN Molecular Diagnostics of Sepsis Study Group
Jun 6, 2016·The American Journal of Emergency Medicine·Christopher W BaughDaniel J Pallin
Jun 28, 2016·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ana I Robles, Curtis C Harris
Jun 30, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Luc GirardAdi F Gazdar
Aug 9, 2016·Journal of Thoracic Disease·Heitor Pons Leite, Lúcio Flávio Peixoto de Lima
Aug 24, 2016·JAMA : the Journal of the American Medical Association·Jethro A HerbergUNKNOWN IRIS Consortium
Sep 10, 2016·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Christopher W BaughDaniel J Pallin
Apr 19, 2017·JAMA : the Journal of the American Medical Association·Myrsini KaforouMichael Levin
Aug 25, 2017·The American Journal of Clinical Nutrition·Kevin BligheJessica A Lasky-Su

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Citations

Jul 22, 2019·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Xuedong ChenShaosong Zhou

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Methods Mentioned

BETA
PCR
metabolomic profiling
EdgeSeq
PCA
blood draw

Software Mentioned

EdgeSeq
LASSO
MetaboAnalyst
IMPaLA
HTG EdgeSeq Parser
RNAseq
profiler
iKnife
GOSt

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