Integrative Pathway Analysis of Metabolic Signature in Bladder Cancer: A Linkage to The Cancer Genome Atlas Project and Prediction of Survival

The Journal of Urology
Friedrich-Carl von RundstedtNagireddy Putluri

Abstract

We used targeted mass spectrometry to study the metabolic fingerprint of urothelial cancer and determine whether the biochemical pathway analysis gene signature would have a predictive value in independent cohorts of patients with bladder cancer. Pathologically evaluated, bladder derived tissues, including benign adjacent tissue from 14 patients and bladder cancer from 46, were analyzed by liquid chromatography based targeted mass spectrometry. Differential metabolites associated with tumor samples in comparison to benign tissue were identified by adjusting the p values for multiple testing at a false discovery rate threshold of 15%. Enrichment of pathways and processes associated with the metabolic signature were determined using the GO (Gene Ontology) Database and MSigDB (Molecular Signature Database). Integration of metabolite alterations with transcriptome data from TCGA (The Cancer Genome Atlas) was done to identify the molecular signature of 30 metabolic genes. Available outcome data from TCGA portal were used to determine the association with survival. We identified 145 metabolites, of which analysis revealed 31 differential metabolites when comparing benign and tumor tissue samples. Using the KEGG (Kyoto Encyclopedia of...Continue Reading

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Citations

Mar 15, 2016·Cancer Treatment Reviews·Francesco MassariRodolfo Montironi
Mar 8, 2016·The Journal of Urology·Ganesh S Palapattu
Feb 3, 2018·Cancer Metastasis Reviews·Ijeoma Adaku UmeloVincent Castronovo
Feb 19, 2019·Expert Review of Proteomics·Chandra Sekhar AmaraNagireddy Putluri
Jun 22, 2019·Metabolomics : Official Journal of the Metabolomic Society·Valentina L KouznetsovaIgor F Tsigelny
Oct 15, 2019·Expert Review of Molecular Diagnostics·Erik KoubaLiang Cheng
Mar 10, 2018·Investigative and Clinical Urology·Xuan-Mei PiaoJayoung Kim
Jul 1, 2020·CNS Oncology·Antonio DonoYoshua Esquenazi
Nov 26, 2019·Analytical Methods : Advancing Methods and Applications·Chandrashekar R AmbatiNagireddy Putluri
Apr 9, 2019·Expert Review of Molecular Diagnostics·Giuseppe LucarelliMichele Battaglia
Jan 16, 2019·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Chandra Sekhar AmaraNagireddy Putluri
Mar 9, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Venkatrao VantakuNagireddy Putluri
Mar 7, 2020·Cancer Letters·Vera Miranda-GonçalvesCarmen Jerónimo

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Methods Mentioned

BETA
metabolomic profiling
amino acid exchange

Software Mentioned

Cytoscape
MassHunter Workstation Software Quantitative Analysis
R

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