Integrator3, a partner of single-stranded DNA-binding protein 1, participates in the DNA damage response

The Journal of Biological Chemistry
Feng ZhangXiaochun Yu


Single-stranded DNA-binding protein 1 (SSB1) plays an important role in the DNA damage response and maintenance of genomic stability. Here, by using protein affinity purification, we have identified Integrator3 (INT3) as a novel partner of SSB1. INT3 forms a complex with SSB1 by constitutively interacting with SSB1 regardless of DNA damage. However, following DNA damage, along with SSB1, INT3 relocates to the DNA damage sites and regulates the accumulation of TopBP1 and BRCA1 there. Moreover, INT3 controls DNA damage-induced Chk1 activation and G(2)/M checkpoint activation. In addition, INT3 is involved in homologous recombination repair by regulating Rad51 foci formation following DNA damage. Taken together, these results demonstrate that INT3 plays a key role in the DNA damage response.


Aug 3, 2010·Acta Pharmacologica Sinica·Leilei ChenXin-yuan Guan
May 8, 2013·Cell Research·Amanda L BainKum Kum Khanna
Jul 3, 2013·Nature Communications·Lin-Yu LuXiaochun Yu
Apr 4, 2013·BMC Molecular Biology·Nicholas W AshtonDerek J Richard
Mar 30, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Isabelle GilbertMarc-André Sirard
May 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Feng ZhangXiaochun Yu
Mar 19, 2014·Cell Reports·Wendan RenHaiwei Song
Apr 29, 2015·Nucleic Acids Research·Ananya KarSandeep Saxena
May 7, 2016·Acta Biochimica Et Biophysica Sinica·Ting Liu, Jun Huang
May 25, 2016·Acta Biochimica Et Biophysica Sinica·Yuanzhong WuTiebang Kang
Jul 20, 2016·Biochimica Et Biophysica Acta·Monica Rienzo, Amelia Casamassimi
May 5, 2017·International Journal of Molecular Sciences·Antonio FedericoAmelia Casamassimi
Jun 3, 2010·Critical Reviews in Biochemistry and Molecular Biology·Rachel Litman Flynn, Lee Zou
Apr 29, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Didier BoucherKum Kum Khanna
May 13, 2020·Briefings in Functional Genomics·Mohd AmirM D Imtaiyaz Hassan
May 16, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthias PfeiferNiklas Feldhahn


Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
Nov 5, 1999·Molecular Cell·M E MoynahanMaria Jasin
Jun 8, 2001·Molecular and Cellular Biology·H Zhao, H Piwnica-Worms
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·A Lopez-GironaP Russell
Nov 1, 2001·Cell Death and Differentiation·Kum Kum KhannaT D Mulhern
Jan 5, 2002·Molecular and Cellular Biology·Kazuhiko YamaneJunjie Chen
Jul 27, 2002·Science·John Rouse, Stephen P Jackson
Mar 18, 2003·Molecular and Cellular Biology·Irene M WardJunjie Chen
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Stephen C West
Jun 5, 2003·Cancer Cell·Jiri Bartek, Jiri Lukas
Jun 7, 2003·Science·Lee Zou, Stephen J Elledge
Oct 25, 2003·Science·Xiaochun YuJunjie Chen
May 18, 2004·Current Opinion in Cell Biology·Michael Lisby, Rodney Rothstein
Jun 11, 2004·Annual Review of Biochemistry·Aziz SancarStuart Linn
Jun 16, 2004·The Journal of Experimental Medicine·Oscar Fernandez-CapetilloAndré Nussenzweig
Jan 5, 2006·Genes & Development·Roger A GreenbergDavid M Livingston
Mar 15, 2006·Cell·Akiko KumagaiWilliam G Dunphy
Mar 15, 2006·DNA Repair·Manuel Stucki, Stephen P Jackson
Aug 29, 2006·Nucleic Acids Research·Ellen FanningAndrew R Nager
Dec 18, 2007·Molecular Cell·J Wade Harper, Stephen J Elledge
May 2, 2008·Nature·Derek J RichardKum Kum Khanna
Jul 3, 2008·Nature Reviews. Molecular Cell Biology·Karlene A Cimprich, David Cortez
Mar 10, 2009·Current Biology : CB·Feng ZhangXiaochun Yu
Jul 17, 2009·The Journal of Biological Chemistry·Yongjiang LiWeidong Wang
Aug 18, 2009·Molecular Cell·Jun HuangJunjie Chen

Related Concepts

SSBP1 gene
FOXN3 wt Allele
Cell Cycle Proteins
Genomic Stability
Base Excision Repair
INTS3 gene
Response to DNA Damage Stimulus
NOTCH4 gene
SSBP1 protein, human
CHEK1 gene

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.