Integrator3, a partner of single-stranded DNA-binding protein 1, participates in the DNA damage response

The Journal of Biological Chemistry
Feng ZhangXiaochun Yu

Abstract

Single-stranded DNA-binding protein 1 (SSB1) plays an important role in the DNA damage response and maintenance of genomic stability. Here, by using protein affinity purification, we have identified Integrator3 (INT3) as a novel partner of SSB1. INT3 forms a complex with SSB1 by constitutively interacting with SSB1 regardless of DNA damage. However, following DNA damage, along with SSB1, INT3 relocates to the DNA damage sites and regulates the accumulation of TopBP1 and BRCA1 there. Moreover, INT3 controls DNA damage-induced Chk1 activation and G(2)/M checkpoint activation. In addition, INT3 is involved in homologous recombination repair by regulating Rad51 foci formation following DNA damage. Taken together, these results demonstrate that INT3 plays a key role in the DNA damage response.

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Related Concepts

SSBP1 gene
FOXN3 wt Allele
Cell Cycle Proteins
Genomic Stability
Base Excision Repair
INTS3 gene
Response to DNA Damage Stimulus
NOTCH4 gene
SSBP1 protein, human
CHEK1 gene

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