Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity

MAbs
Daniel F LuscheDavid R Soll

Abstract

Breast cancer, melanoma and glioblastoma cells undergo cell-mediated aggregation and aggregate coalescence in a transparent 3D Matrigel environment. Cells from normal tissue and non-tumorigenic cell lines do not exhibit these behaviors. Here, 266 monoclonal antibodies (mAbs) demonstrated to interact with a wide variety of membrane, secreted and matrix proteins, have been screened for their capacity to block these tumorigenic cell-specific behaviors in a 3D environment. Remarkably, only six of the 266 tested mAbs exhibited blocking activity, four targeting integrin ß-1, one targeting integrin α-3 and one targeting CD44. Colocalization of integrins ß-1 and α-3 in fixed cells and in live aggregates suggests that the integrin α-3 ß-1 dimer plays a central role in cancer cell aggregation in the 3D environment provided by Matrigel. Our results suggest that blocking by anti-integrin and anti-CD44 mAbs involves interference in cell-cell interactions.

References

Sep 27, 1979·Nature·M Abercrombie
Mar 1, 1992·Clinical & Experimental Metastasis·K R GehlsenP Sriramarao
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·O W PetersenM J Bissell
Mar 1, 1990·The Journal of Cell Biology·H LarjavaK M Yamada
Aug 13, 1990·Biochimica Et Biophysica Acta·A YamaguchiH Yamazaki
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R PytelaE Ruoslahti
Nov 3, 1989·Science·A B Pardee
Dec 1, 1988·Nature·A SonnenbergF Hogervorst
Apr 1, 1985·The Journal of Cell Biology·W T ChenK M Yamada
Sep 1, 1974·Journal of the National Cancer Institute·R CailleauW J Reeves
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Oct 9, 1980·The New England Journal of Medicine·M B Sporn, G J Todaro
Jan 1, 1994·Cold Spring Harbor Symposia on Quantitative Biology·S W LoweD E Housman
May 26, 1995·The Journal of Biological Chemistry·P E HughesM H Ginsberg
Jul 1, 1993·The Journal of Cell Biology·R J PeachA Aruffo
Jan 1, 1997·Advances in Cancer Research·D NaorD Ish-Shalom
Feb 7, 1998·The Journal of Biological Chemistry·J BajorathA Aruffo
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Mar 1, 2000·Molecular Pathology : MP·S GoodisonD Tarin
Jan 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M S KurtisR L Sah
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·W Knudson, R F Loeser
May 1, 1954·Experimental Cell Research·M ABERCROMBIE, J E HEAYSMAN
Feb 1, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Bruce R Smoller
Aug 19, 2007·Methods in Enzymology·E A Wayner, B G Hoffstrom
Jul 23, 2008·Molecular and Cellular Biology·Jia-Lin LeeJeou-Yuan Chen

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Citations

May 30, 2019·Cancers·Imjoo Jang, Karen A Beningo

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Methods Mentioned

BETA
confocal microscopy
ELISA

Software Mentioned

J3D
DIAS

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