Integrin α11 in pancreatic stellate cells regulates tumor stroma interaction in pancreatic cancer

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Jonas SchnittertJai Prakash

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is the deadliest tumor due to its highly abundant tumor stroma. Pancreatic stellate cells (PSCs) are considered precursor cells of cancer-associated fibroblasts (CAFs), which induce tumor progression, invasion, and metastasis. In this study, we investigated the role of integrin subunit α (ITGA) 11, the receptor for collagen type I, in tumor stroma interaction. Clinical sample analysis showed that ITGA11 was overexpressed by CAFs in PDAC stroma, as shown with colocalization immunostaining with α-smooth muscle actin. In contrast, there was no expression in healthy pancreas. Public transcriptomic data confirmed a reduced expression of ITGA11 in healthy pancreas and adjacent nontumoral tissues compared with human tumor tissues. Primary human PSCs (hPSCs) activated with either TGF-β or pancreatic cancer cell (PANC-1)-conditioned medium (CM) resulted in the significant up-regulation of ITGA11 and various CAF markers. Furthermore, short hairpin RNA (shRNA)-mediated stable ITGA11 knockdown (shITGA11) in hPSCs significantly inhibited TGF-β- and PANC-1 CM-mediated activation at both gene and protein levels of extracellular matrix, cytokines, and adhesion molecules. Additionally, shITGA11 hPSCs had ...Continue Reading

References

Jul 25, 2006·Nature Reviews. Cancer·Andrew I Minchinton, Ian F Tannock
Feb 5, 2008·Cancer Research·Rosa F HwangCraig D Logsdon
Apr 3, 2008·Cancer Research·Alain VonlaufenMinoti V Apte
Nov 17, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Ning LuDonald Gullberg
May 13, 2011·The New England Journal of Medicine·Thierry ConroyUNKNOWN PRODIGE Intergroup
Jun 15, 2011·Annual Review of Cell and Developmental Biology·Johanna Ivaska, Jyrki Heino
Jun 15, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ruchi BansalJai Prakash
Dec 23, 2011·The Biochemical Journal·Mario A ShieldsHidayatullah G Munshi
Feb 15, 2012·Journal of Gastroenterology and Hepatology·Minoti V Apte, Jeremy S Wilson
Oct 2, 2012·Molecular Cancer Research : MCR·George S KaragiannisEleftherios P Diamandis
Apr 30, 2013·Gastroenterology·Minoti V ApteStephen J Pandol
Jul 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Albrecht NeesseDavid A Tuveson
Aug 7, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Geng ZhangS Perwez Hussain
Oct 18, 2013·The New England Journal of Medicine·Daniel D Von HoffMarkus F Renschler
Jan 1, 2010·Cancers·Hansjörg HabischMax G Bachem
Jan 13, 2015·Trends in Cell Biology·Laetitia SeguinDavid A Cheresh
May 20, 2015·Nature Cell Biology·Bruno Costa-SilvaDavid Lyden
May 23, 2015·Science Translational Medicine·Nilgun I ReedDean Sheppard
Jun 27, 2015·Genes & Diseases·Dacheng Xie, Keping Xie
Feb 3, 2016·Frontiers in Oncology·Praneeth R KunintyJai Prakash
May 4, 2016·Cell and Tissue Research·Kylie P ConroyNeil C Henderson
Jul 5, 2016·Scientific Reports·Muge SarperArmando Del Río Hernández
Aug 24, 2016·Nature Reviews. Cancer·Raghu Kalluri
Oct 5, 2016·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Himalaya ParajuliDaniela Elena Costea
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 25, 2017·The Journal of Experimental Medicine·Daniel ÖhlundDavid A Tuveson
Jul 20, 2017·Advanced Drug Delivery Reviews·Saleh YazdaniJai Prakash
Jul 26, 2017·Trends in Cancer·Jai Prakash
Nov 18, 2017·Experimental & Molecular Medicine·Ruchi BansalJai Prakash

❮ Previous
Next ❯

Citations

May 30, 2019·Cancers·Imjoo Jang, Karen A Beningo
Nov 26, 2020·International Journal of Molecular Sciences·Justin F CreedenRobert E McCullumsmith
Dec 19, 2020·Journal of Oncology·Jun-Fu WangJun-Qiang Chen
Mar 7, 2021·Cancers·Teresa Gagliano, Claudio Brancolini
Aug 23, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Matthew HaddenYogambha Ramaswamy
May 22, 2021·Journal of Experimental & Clinical Cancer Research : CR·Freja Albjerg VenningChris Denis Madsen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.