Abstract
The T-2 toxin exerts a variety of toxic effects on both experimental animals and humans. The integrin family plays a major role in mediating cell-ECM interactions. Therefore, the present study aimed to investigate the involvement of integrin α2β1 in T-2 toxin-induced C28/I2 chondrocyte damage. The pathological damage of articular cartilage injury induced by T-2 toxin was observed by H&E staining. The expression levels of collagen 2 and MMP-13 (Matrix metalloproteinases 13) were detected using immunohistochemistry in articular cartilage tissues and western blotting in the cells. The blocking effect of integrin α2β1 inhibitor on T-2 toxin-induced chondrocyte matrix degradation was examined by western blotting. Further, the effect of integrin α2β1 inhibitor on T-2 toxin-induced chondrocyte apoptosis was analyzed. About 100 ng/g body weight (BW)/day T-2 toxin was fed to Sprague-Dawley (SD) rats, T-2 toxin treatment (0, 6, 12, and 24 ng/mL) induced C28/I2 chondrocytes. Both in vivo and in vitro, chondrocyte survival was inhibited, and the production of type II collagen was significantly reduced (p < 0.05). However, the level of MMP-13 was up-regulated (p < 0.05). Matrix degradation was effectively blocked after the pre-treatment by i...Continue Reading
References
Apr 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D M SalterM S Gourlay
Dec 1, 1995·The Journal of Cell Biology·O LangholzB Eckes
Aug 11, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D L ReidJ Mollenhauer
Feb 24, 2001·European Journal of Cell Biology·P ZigrinoC Mauch
Oct 31, 2001·Cell and Tissue Research·L CamperE Lundgren-Akerlund
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·W Knudson, R F Loeser
May 6, 2003·Circulation Research·Robert Visse, Hideaki Nagase
Nov 26, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jinghong ChenZhilun Wang
Apr 26, 2006·Journal of Cell Science·Zhi-Gang ZhangMonique Aumailley
May 31, 2008·The American Journal of Pathology·Valeria M DejicaA Robin Poole
Feb 26, 2009·Genes & Development·Kyle R LegateReinhard Fässler
Jul 20, 2010·Amino Acids·T Klein, R Bischoff
Aug 14, 2010·International Journal of Rheumatic Diseases·Denglu YanFu-Xing Pei
Nov 16, 2011·Arthritis and Rheumatism·Marvin A PetersThomas Pap
Jan 20, 2012·Rheumatology International·Fang GuanClare Hughes
Dec 7, 2013·PloS One·Vanessa L S LaPointeMolly M Stevens
Aug 12, 2014·Osteoarthritis and Cartilage·X GuoM J Lammi
Aug 30, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Richard F Loeser
Jul 16, 2016·Biochemical and Biophysical Research Communications·Xiaoqing WangXiong Guo
Citations
Jun 18, 2021·Toxicon : Official Journal of the International Society on Toxinology·Hui WangJinghong Chen
Jun 17, 2021·Cartilage·Meng ZhangJinghong Chen
Oct 22, 2021·Toxicology·Yi-Nan LiuJing-Hong Chen