Integrin α2β1-targeting ferritin nanocarrier traverses the blood-brain barrier for effective glioma chemotherapy.

Journal of Nanobiotechnology
Chiun-Wei HuangFeng-Ting Huang

Abstract

Ferritin, the natural iron storage protein complex, self-assembles into a uniform cage-like structure. Human H-ferritin (HFn) has been shown to transverse the blood-brain barrier (BBB) by binding to transferrin receptor 1 (TfR1), which is abundant in endothelial cells and overexpressed in tumors, and enters cells via endocytosis. Ferritin is easily genetically modified with various functional molecules, justifying that it possesses great potential for development into a nanocarrier drug delivery system. In this study, a unique integrin α2β1-targeting H-ferritin (2D-HFn)-based drug delivery system was developed that highlights the feasibility of receptor-mediated transcytosis (RMT) for glioma tumor treatment. The integrin targeting α2β1 specificity was validated by biolayer interferometry in real time monitoring and followed by cell binding, chemo-drug encapsulation stability studies. Compared with naïve HFn, 2D-HFn dramatically elevated not only doxorubicin (DOX) drug loading capacity (up to 458 drug molecules/protein cage) but also tumor targeting capability after crossing BBB in an in vitro transcytosis assay (twofold) and an in vivo orthotopic glioma model. Most importantly, DOX-loaded 2D-HFn significantly suppressed subcuta...Continue Reading

References

Apr 1, 1978·Physiological Reviews·H N Munro, M C Linder
Aug 15, 1992·Journal of Inorganic Biochemistry·S C AndrewsS J Yewdall
Jun 1, 1973·The Biochemical Journal·R R Crichton, C F Bryce
Jan 1, 1996·Archives of Biochemistry and Biophysics·S StefaniniE Chiancone
Feb 22, 2008·Current Drug Targets·K D McCall-Culbreath, M M Zutter
Aug 2, 2008·Neoplasia : an International Journal for Oncology Research·Christopher L HallKenneth L van Golen
Dec 20, 2008·Lymphatic Research and Biology·Barbara Garmy-Susini, Judith A Varner
Aug 25, 2009·The American Journal of Pathology·James D San AntonioRenato V Iozzo
Dec 24, 2009·Nature Reviews. Cancer·Jay S Desgrosellier, David A Cheresh
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Li LiWilliam E Seaman
Sep 29, 2012·Current Pharmaceutical Biotechnology·Changyou Zhan, Weiyue Lu
Feb 26, 2013·Nanoscale Research Letters·Abolfazl AkbarzadehKazem Nejati-Koshki
Apr 4, 2013·The Journal of Biological Chemistry·Stephanie PfaffenMichael E P Murphy
Jul 11, 2013·International Journal of Cell Biology·Guangqing Xiao, Liang-Shang Gan
May 6, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kinam Park
Jul 16, 2014·Advances in Experimental Medicine and Biology·Aasakiran MadamanchiMary M Zutter
Jul 25, 2014·European Journal of Pharmacology·Shaloam Dasari, Paul Bernard Tchounwou
Aug 15, 2014·Archives of Toxicology·Dario Finazzi, Paolo Arosio
Aug 28, 2014·Beilstein Journal of Nanotechnology·Amanee D SalaamDerrick Dean
Nov 25, 2014·Chemical Reviews·Kourosh Honarmand EbrahimiWilfred R Hagen
Aug 25, 2015·Seminars in Cancer Biology·Dalila NaciFawzi Aoudjit
Nov 1, 2015·The Biochemical Journal·Paolo ArosioMaura Poli
Feb 4, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maha HussainUNKNOWN PERSEUS Study Group
Mar 13, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Marta TruffiSerena Mazzucchelli
Dec 13, 2016·Nanoscale·Valeria de TurrisPaola Baiocco
Oct 19, 2017·International Journal of Nanomedicine·Fakhar Ud DinAlam Zeb
Mar 22, 2018·Signal Transduction and Targeted Therapy·Sudipta SenapatiPralay Maiti
Jul 17, 2018·Advanced Drug Delivery Reviews·Susanne K GolombekTwan Lammers
Mar 10, 2019·Nature Communications·Linda Celeste MontemiglioBeatrice Vallone
Jul 25, 2019·Cancers·Begoña Alday-ParejoCurzio Rüegg
Dec 16, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wei LiuLing Zhang

❮ Previous
Next ❯

Software Mentioned

Octet RED96
Octet DataAnalysis
ModFit LT
GeneMapper

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Related Papers

Journal of Controlled Release : Official Journal of the Controlled Release Society
Wei LiuLing Zhang
American Journal of Physiology. Cell Physiology
J FisherJames R Connor
Journal of Controlled Release : Official Journal of the Controlled Release Society
Jiuyang HeXiyun Yan
© 2021 Meta ULC. All rights reserved