Integrin β3 is not critical for neutrophil recruitment in a mouse model of pneumococcal pneumonia

Cell and Tissue Research
Kyathanahalli S JanardhanBaljit Singh

Abstract

Streptococcus pneumoniae is one of the most common causes of bacterial pneumonias in humans. Neutrophil migration into lungs infected with S. pneumoniae is central to the host defense but the mechanisms of neutrophil recruitment, as mediated by S. pneumoniae, into lungs are incompletely understood. Therefore, we have assessed the role of integrin αvβ3 by evaluating its subunit β3 in a mouse model of lung inflammation induced by S. pneumonia. Integrin subunit β3 knockout (β3(-/-)) and wild-type (WT) mice were intratracheally instilled with either S. pneumoniae or saline. Other groups of WT mice were treated intraperitoneally with 25 μg or 50 μg of antibody against integrin β3 or with isotype-matched antibody at 1 h before instillation of S. pneumoniae. Mice were killed 24 h after infection. Flow cytometry confirmed the absence or presence of integrin subunit β3 on peripheral blood neutrophils in β3(-/-) or WT mice, respectively. Neutrophil numbers in bronchoalveolar lavage (BAL) from infected β3(-/-) and WT mice showed no differences. Neutrophil numbers in BAL of infected WT mice treated with β3 antibody were lower compared with those without antibody but similar to those of mice administered isotype-matched antibody. Many neutr...Continue Reading

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Citations

Apr 23, 2013·Cellular Microbiology·Bas G J SurewaardCarla J C de Haas
Jul 10, 2012·International Journal of Medical Microbiology : IJMM·Younho ChoiSangryeol Ryu
Dec 19, 2017·Cell and Tissue Research·Gurpreet Kaur Aulakh
Feb 6, 2021·Cellular and Molecular Life Sciences : CMLS·Wan-Chi Lin, Michael B Fessler

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