Integrin adhesion in regulation of lacrimal gland acinar cell secretion

Experimental Eye Research
Sofia V AnderssonJ Peter Gierow

Abstract

The extracellular microenvironment regulates lacrimal gland acinar cell secretion. Culturing isolated rabbit lacrimal gland acinar cells on different extracellular matrix proteins revealed that laminin enhances carbachol-stimulated secretion to a greater extent than other extracellular matrix proteins investigated. Furthermore, immunofluorescence indicated that integrin subunits, potentially functioning as laminin receptors are present in acinar cells. Among these, the integrin alpha6 and beta1 subunit mRNA expression was also confirmed by RT-PCR and sequence analysis. Secretion assays, which measured beta-hexosaminidase activity released in the culture media, demonstrated that function-blocking integrin alpha6 and beta1 monoclonal antibodies (mAbs) induce a rapid, transient and dose-dependent secretory response in cultured cells. To determine the intracellular pathways by which integrin alpha6 and beta1 mAbs could induce secretion, selected second messenger molecules were inhibited. Although inhibitors of protein kinase C and IP(3)-induced Ca(2+) mobilization attenuated carbachol-stimulated secretion, no effect on integrin mAb-induced release was observed. In addition, protein tyrosine kinases do not appear to have a role in t...Continue Reading

References

Apr 1, 1990·The Journal of Cell Biology·C H Streuli, M J Bissell
Mar 1, 1994·Molecular and Cellular Biology·M D SchallerJ T Parsons
Mar 28, 1995·Biochemical and Biophysical Research Communications·R W Wrenn, L E Herman
Jun 1, 1995·Experimental Cell Research·A S Menko, N J Philip
Mar 1, 1994·The Journal of Cell Biology·M L Matter, G W Laurie
Nov 1, 1996·The American Journal of Physiology·J P GierowA K Mircheff
Mar 1, 1997·Experimental Eye Research·L S Grushkin-LernerV Trinkaus-Randall
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·X ZhangS K Hanks
Aug 5, 1999·Cellular Signalling·H SchöttelndreierA H Guse
Aug 14, 1999·Science·F G Giancotti, E Ruoslahti
Jan 6, 2000·Nature Cell Biology·F G Giancotti
Sep 16, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A C Erickson, J R Couchman
Jul 31, 2001·Biochimica Et Biophysica Acta·M D Schaller
Dec 1, 2001·Cellular Signalling·H SchöttelndreierA H Guse
Mar 5, 2003·Advances in Experimental Medicine and Biology·J Peter GierowElsie C Sjögren
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Karl S MatlinAnna Zuk
Jan 10, 2004·International Review of Cytology·Robin R Hodges, Darlene A Dartt
Jan 23, 2004·American Journal of Physiology. Endocrinology and Metabolism·Jingsong LiGuoDong Li
May 26, 2004·Biochemical Society Transactions·R L JulianoA Aplin
Jun 11, 2004·Cell Communication and Signaling : CCS·Twanda L ThirkillGordon C Douglas
Oct 14, 2004·The Journal of Cell Biology·DeannaLee M BeauvaisAlan C Rapraeger
Oct 27, 2004·Advances in Protein Chemistry·Timothy A Springer, Jia-Huai Wang
Dec 3, 2004·Molecular Biology of the Cell·Wei YuMirjam M P Zegers

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Citations

Apr 21, 2009·Progress in Retinal and Eye Research·Darlene A Dartt

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