Integrin beta 8 (ITGB8) regulates embryo implantation potentially via controlling the activity of TGF-B1 in mice

Biology of Reproduction
Vijay KumarRajesh Kumar Jha

Abstract

Integrins (ITGs) are mediators of cell-cell and cell-matrix interactions, which are also associated with embryo implantation processes by controlling the interaction of blastocyst with endometrium. During early pregnancy, ITGbeta8 (ITGB8) has been shown to interact with latent transforming growth factor (TGF) beta 1 (TGFB1) at the fetomaternal interface. However, the precise role of ITGB8 in the uterus and its association with embryo implantation has not been elucidated. Therefore, we attempted to ascertain the role of ITGB8 during the window of embryo implantation process by inhibiting its function or protein expression. Uterine plasma membrane-anchored ITGB8 was augmented at peri-implantation and postimplantation stages. A similar pattern of mRNA expression was also found during the embryo implantation period. An immunolocalization study revealed the presence of ITGB8 on luminal epithelial cells along with mild expression on the stromal cells throughout the implantation period studied; however, an intense fluorescence was noted only during the peri- and postimplantation stages. Bioneutralization and mRNA silencing of the uterine Itgb8 at preimplantation stage reduced the rate/frequency of embryo implantation and subsequent pr...Continue Reading

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Citations

Jan 24, 2016·Molecular and Cellular Endocrinology·Sangappa Basanna ChadchanRajesh Kumar Jha
Nov 20, 2016·Journal of Cellular and Molecular Medicine·Chao-Yang MengJing-Pian Peng
Mar 3, 2017·Current Protocols in Nucleic Acid Chemistry·Jon D Moulton
Jan 4, 2017·Journal of Cell Science·John D Aplin, Peter T Ruane
Sep 25, 2016·Reproductive Sciences·Vaibhave UbbaRajesh Kumar Jha
Dec 14, 2017·International Journal of Molecular Sciences·Joanna BudnaBartosz Kempisty
Jun 15, 2018·Biology of Reproduction·Vinay ShuklaAnila Dwivedi
May 1, 2017·Reproductive Sciences·Vaibhave UbbaRajesh Kumar Jha
May 30, 2021·Molecular Human Reproduction·Upendra Kumar SoniRajesh Kumar Jha

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Methods Mentioned

BETA
protein assay
PCR

Software Mentioned

Totallab Quant
GraphPad Prism

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