Integrin-dependent control of inositol lipid synthesis in vascular endothelial cells and smooth muscle cells

Experimental Cell Research
H P McNameeD E Ingber

Abstract

Extracellular matrix (ECM) molecules, such as fibronectin (FN), regulate fibroblast sensitivity to soluble growth factors, in part, by controlling cellular levels of phosphatidylinositol bis-phosphate (PIP2), the substrate for phospholipase C-gamma (McNamee et al., 1993, J. Cell Biol. 121, 673-678). In the present study, we extended these investigations by exploring whether cells of the vascular wall also exhibit this response and analyzing the mechanism by which adhesion to ECM regulates intracellular PIP2 mass. Capillary endothelial cells, pulmonary vascular smooth muscle cells, and C3H 101/2 fibroblasts were all found to exhibit a similar two- to threefold increase in PIP2 mass within 3 h after binding to dishes coated with FN. Furthermore, similar effects were observed using dishes coated with a variety of different ECM molecules, including collagen types I and IV as well as a synthetic RGD-containing peptide. An increase in PIP2 mass also was produced when suspended cells bound to microbeads (4.5 micron diameter; coated with RGD-peptide or anti-integrin beta 1 antibody) that induce local integrin clustering and focal adhesion formation, independently of cell spreading. In contrast, neither binding of soluble FN nor binding...Continue Reading

Citations

Jan 1, 1997·Annual Review of Physiology·D E Ingber
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Guillermina M GoñiDaniel Lietha
Sep 18, 2012·Cell Adhesion & Migration·Christine Lawson, David D Schlaepfer
Sep 24, 2004·American Journal of Physiology. Cell Physiology·Hayden HuangRichard T Lee
Oct 10, 2007·Biochemistry. Biokhimii︠a︡·N V ProkazovaE R Andreeva
Oct 14, 2005·American Journal of Physiology. Cell Physiology·Madalina Condrescu, John P Reeves
Sep 1, 2006·The Journal of Biological Chemistry·Masaya YamamotoHelen L Yin
Feb 9, 2016·Journal of Animal Physiology and Animal Nutrition·J SchmeisserF Fru-Nji
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D Ingber
Jan 1, 1998·Journal of Cellular Biochemistry·Donald Ingber
Nov 12, 2020·International Journal of Molecular Sciences·Kalpana Mandal
Feb 13, 2001·Biochemical and Biophysical Research Communications·M E Dunlop, E E Muggli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.