PMID: 7537275May 1, 1995Paper

Integrin-dependent translocation of phosphoinositide 3-kinase to the cytoskeleton of thrombin-activated platelets involves specific interactions of p85 alpha with actin filaments and focal adhesion kinase

The Journal of Cell Biology
C GuinebaultH Chap

Abstract

Thrombin-induced accumulation of phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) but not of PtdIns(3,4,5,)P3 is strongly correlated with the relocation to the cytoskeleton of 29% of the p85 alpha regulatory subunit of phosphoinositide 3-kinase (PtdIns 3-kinase) and is accompanied by a significant increase in PtdIns 3-kinase activity in this subcellular fraction. Actually, PtdIns(3,4)P2 accumulation and PtdIns 3-kinase, pp60c-src, and p125FAK translocations as well as aggregation were concomitant events occurring with a distinct lag after actin polymerization. The accumulation of PtdIns(3,4)P2 and the relocalization of PtdIns 3-kinase to the cytoskeleton were both dependent on tyrosine phosphorylation, integrin signaling, and aggregation. Furthermore, although p85 alpha was detected in anti-phosphotyrosine immunoprecipitates obtained from the cytoskeleton of thrombin-activated platelets, we failed to demonstrate tyrosine phosphorylation of cytoskeletal p85 alpha. Tyrphostin treatment clearly reduced its presence in this subcellular fraction, suggesting a physical interaction of p85 alpha with a phosphotyrosyl protein. These data led us to investigate the proteins that are able to interact with PtdIns 3-kinase in the cytosk...Continue Reading

References

Nov 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G MyersM F White
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Ferrell, G S Martin
Jul 1, 1970·British Journal of Haematology·N G ArdlieJ F Mustard
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·H C Chen, J L Guan
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M TortiC Balduini
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K V PrasadC E Rudd
Jan 15, 1993·Biochemical and Biophysical Research Communications·E André, M Becker-André
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Feb 6, 1998·Journal of Neuroscience Research·M M BergW L Klein
May 1, 2001·Journal of Cellular Biochemistry·S MukhopadhyayD Dash
Jun 30, 2005·Cancer Metastasis Reviews·Silke KuphalAnja-Katrin Bosserhoff
Sep 29, 2000·Biochemical Pharmacology·B PayrastreH Chap
Jun 1, 2001·Cellular Signalling·B PayrastreM Gratacap
Oct 1, 1996·Current Opinion in Cell Biology·J A Varner, D A Cheresh
Aug 1, 1997·The International Journal of Biochemistry & Cell Biology·J L Guan
Feb 1, 2003·Journal of Neurochemistry·Samantha MessinaFabio Benfenati
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·T R Polte, S K Hanks
Feb 4, 2011·Molecular Biology of the Cell·Therese B DeramaudtPhilippe Rondé
Jun 17, 2000·The EMBO Journal·J J MartinezS J Hultgren
Nov 5, 2002·Clinical and Diagnostic Laboratory Immunology·Madhumita Sirkar, Subrata Majumdar
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Burridge, M Chrzanowska-Wodnicka
Dec 6, 2000·The Journal of Clinical Investigation·M SumitomoD M Nanus
Mar 20, 2014·PloS One·Thérèse B DeramaudtPhilippe Rondé
Nov 2, 2001·Biochemical and Biophysical Research Communications·F PaulheC Racaud-Sultan
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·A D MundayC A Mitchell
Nov 17, 2015·Marine Drugs·Emmanuel AmpofoMatthias W Laschke

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