Integrin-induced tyrosine phosphorylation of protein-tyrosine phosphatase-alpha is required for cytoskeletal reorganization and cell migration

The Journal of Biological Chemistry
Min ChenCatherine J Pallen

Abstract

Protein-tyrosine phosphatase-alpha (PTPalpha) activates Src family kinases (SFKs) to promote the integrin-stimulated early autophosphorylation of focal adhesion kinase (FAK). We report here that integrin stimulation induces tyrosine phosphorylation of PTPalpha. PTPalpha was dephosphorylated upon fibroblast detachment from the substratum and rephosphorylated when cells were plated on the integrin ligand fibronectin. alpha PTP phosphorylation occurred at Tyr789 and required SFKs (Src or Fyn/Yes), FAK, and an intact cytoskeleton. It also required active PTPalpha or constitutively active Src. These observations indicate that PTPalpha activates SFKs and that the subsequently activated SFK.FAK tyrosine kinase complex in turn phosphorylates PTPalpha. Reintroduction of wild-type PTPalpha or unphosphorylatable PTPalpha(Y789F) (but not inactive PTPalpha) into PTPalpha-null fibroblasts restored defective integrin-induced SFK activation, FAK phosphorylation, and paxillin phosphorylation. PTPalpha(Y789F) and inactive PTPalpha could not rescue delayed actin stress fiber assembly and focal adhesion formation or defective cell migration. This study distinguishes two roles of PTPalpha in integrin signaling: an early role as an activator of SFKs...Continue Reading

References

Mar 1, 1994·Molecular and Cellular Biology·M D SchallerJ T Parsons
May 16, 1998·The Journal of Biological Chemistry·V BhandariC J Pallen
Mar 19, 1999·Molecular and Cellular Biology·E S OhS M Thomas
May 6, 1999·The EMBO Journal·R A KlinghofferP Soriano
Dec 14, 1999·The Journal of Biological Chemistry·J W ThomasM D Schaller
Mar 4, 2000·The EMBO Journal·X M ZhengD Shalloway
Nov 2, 2001·The EMBO Journal·X M Zheng, D Shalloway
Apr 16, 2003·The Journal of Cell Biology·Gotz von WichertMichael P Sheetz
Nov 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elena G Arias-SalgadoSanford J Shattil
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Dec 31, 2003·Developmental Biology·Myrto Raftopoulou, Alan Hall
Jan 28, 2004·Cell·Keith Burridge, Krister Wennerberg
Oct 19, 2004·Oncogene·Martin P Playford, Michael D Schaller
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Satyajit K MitraDavid D Schlaepfer

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Citations

Jan 31, 2008·Cancer Metastasis Reviews·Dalia Berman-GolanAri Elson
Apr 14, 2010·Molecular and Cellular Biology·Andrei M Vacaru, Jeroen den Hertog
Jul 17, 2013·Molecular Neurodegeneration·Hansen WangGerold Schmitt-Ulms
Aug 14, 2013·PloS One·Dhaarmini RajshankarChristopher A McCulloch
Sep 6, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sami Ben HamidaDorit Ron
Apr 4, 2014·Molecular Biology of the Cell·Eynat FinkelshteinAri Elson
Apr 25, 2007·Cell Communication & Adhesion·Gareth Owain EdwardsW Terence Coakley
Aug 22, 2006·Current Opinion in Cell Biology·Satyajit K Mitra, David D Schlaepfer
Mar 28, 2013·Acta Biochimica Et Biophysica Sinica·Jiamin WangXinmin Zheng
May 3, 2008·Journal of Cellular Biochemistry·Qiu-Yan WangHui-Li Chen
Apr 14, 2009·Molecular Reproduction and Development·Jinping LuoWilliam H Kinsey
Jul 9, 2009·Journal of Biomedical Materials Research. Part a·Ian S MillerWilliam M Gallagher
Jan 25, 2008·American Journal of Physiology. Cell Physiology·Maria Teresa Herrera AbreuGregory P Downey
Jun 16, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Barbara Tremper-WellsDavid Shalloway
Jul 12, 2018·Journal of Cell Science·Philip T T LyCatherine J Pallen
May 1, 2015·Oncotarget·Qurratulain AftabChristian C Naus
Dec 3, 2016·Biochemical Society Transactions·Maayan BarneaAri Elson
May 9, 2015·Journal of Neurochemistry·Jian XuPaul J Lombroso
Aug 13, 2013·Periodontology 2000·Yongqiang WangChristopher A McCulloch
Mar 13, 2021·The Biochemical Journal·Katherine A YoungHayley J Sharpe

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