PMID: 12757195May 22, 2003Paper

Integrin-mediated preadipocyte adhesion and migration on laminin-1

Annals of Biomedical Engineering
C W Patrick, Xuemei Wu

Abstract

Cell adhesion and migration are key events in many biological processes and depend on extracellular matrix proteins. Understanding these cellular events in adipogenesis is paramount to elucidating the biological mechanisms underlying preadipocyte-specific aspects of obesity, diabetes, and adipose tissue development for the design of pharmaceutical screening and tissue engineering strategies. We quantitatively investigated preadipocyte adhesion and migration on laminin-1 surfaces and identified candidate cognate preadipocyte receptors for laminin-1. In adhesion studies, we found that preadipocytes readily adhered to laminin-1 as compared with other extracellular matrix proteins. In addition, immunocytochemical analysis demonstrated that an array of integrin molecules was present on the surface of preadipocytes. Preadipocyte adhesion on laminin-1 was quantitatively assessed using a sedimentation adhesion assay, and results suggested that preadipocyte adhesion to laminin-1 was mediated by the alpha1beta1 integrin. In addition, digital time-lapse microscopy and quantitative cell tracking revealed that inhibition of the alpha1beta1 integrin resulted in abrogation of preadipocyte migration on laminin-1 surfaces. These results strongl...Continue Reading

Citations

Nov 19, 2003·Clinics in Plastic Surgery·Elisabeth K BeahmCharles W Patrick
Nov 2, 2005·Tissue Engineering·Parul Natvar PatelCharles W Patrick
Sep 2, 2008·Tissue Engineering. Part a·Wakako TsujiMasakazu Toi
Jul 1, 2010·Stem Cell Research & Therapy·Jeffrey M GimbleBruce A Bunnell
Oct 24, 2008·Molecular Endocrinology·Lily C ChaoPeter Tontonoz
Dec 15, 2010·Biochemical and Biophysical Research Communications·José Romo-YáñezLuis A Salazar-Olivo
Jul 6, 2010·Experimental Cell Research·Soon-Mi LimAndreea Trache
Feb 23, 2010·Biomaterials·Ferdinand P BrandlAchim M Göpferich
Dec 17, 2009·Journal of Tissue Viability·Yu-Chieh ChiuEric M Brey
Jul 29, 2008·Medical Image Analysis·Kang LiPhil G Campbell
Aug 6, 2013·Journal of Biomedical Materials Research. Part a·Esra KorurerErdal Karaoz
Sep 29, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Shomron Ben-Horin, Ilan Bank
Nov 4, 2004·The Journal of Surgical Research·Chad LaurichKelli M Bullard
Jul 17, 2004·Annual Review of Biomedical Engineering·Charles W Patrick
Nov 7, 2016·Tissue Engineering. Part a·Yu-Sheng HsuehFeng-Huei Lin
Oct 4, 2006·Plastic and Reconstructive Surgery·Julio A Clavijo-AlvarezKacey G Marra
Jan 18, 2006·Molecular Nutrition & Food Research·Yung-Hsi KaoChia-Lin Chen
Jun 7, 2006·The Journal of Biological Chemistry·Johannes A EbleSusanne Grässel
Dec 30, 2016·The Journal of Biological Chemistry·Tomoko KaburagiNaoyuki Taniguchi
Jan 28, 2021·Scientific Reports·Vladimir V StrelnikovOlga A Simonova

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Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

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