Integrin-mediated transfection with peptides containing arginine-glycine-aspartic acid domains

Gene Therapy
Stephen L HartJ W Fabre

Abstract

Two synthetic peptides comprising an RGD moiety for integrin binding and a polylysine moiety for DNA binding were tested for transfection efficiency under a variety of different conditions. Binding of target cells to the peptide was shown to be strongly dependent on cyclisation of the peptides via cysteine residues. Low (10 microM) concentrations of chloroquine, added to assist endocytic exit, unexpectedly reduced transfection efficiency in two of the cell lines tested. COS-7 and ECV304. However, transfection efficiency increased at higher chloroquine concentrations and exceeded that in the absence of chloroquine in the case of the COS-7 and A375M cell lines. With the ECV304 cell line, optimum transfection occurred in the absence of chloroquine. Transfection efficiency of the peptides was greatest at peptide:DNA ratios of 4:1 (w/w), which were calculated to generate complexes containing approximately 5000 peptide molecules per plasmid. This represented approximately a 6:1 ratio of positive to negative charges. Peptide 5 was shown to have a higher transfection efficiency under most conditions, possibly because of more efficient stabilisation of cyclisation by two cysteine-cysteine bonds.

Citations

Feb 24, 2001·The Journal of Gene Medicine·J FominayaA Bernad
Apr 25, 2001·The Journal of Gene Medicine·E S ScottW H Colledge
Jul 5, 2001·The Journal of Gene Medicine·S PatelJ W Fabre
Jul 12, 2002·The Journal of Gene Medicine·Richard ParkesStephen L Hart
Sep 13, 2001·Mental Retardation and Developmental Disabilities Research Reviews·T D Weibel, R O Brady
Feb 4, 2010·Cell Biology and Toxicology·Stephen L Hart
Feb 11, 2004·Progress in Biophysics and Molecular Biology·Andrew H Baker
Nov 10, 2000·Advanced Drug Delivery Reviews·R J Parkes, S L Hart
Nov 24, 2001·Advanced Drug Delivery Reviews·G ZuberJ P Behr
Sep 27, 2000·Biochimica Et Biophysica Acta·L VaysseB Arveiler
Aug 6, 2011·Molecular Pharmaceutics·Laila KudsiovaM Jayne Lawrence
Jan 9, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Scott A IrvineStephen L Hart
May 4, 2001·Anti-cancer Drugs·A G Schatzlein
Jan 25, 2002·Medicine·Danuta Balicki, Ernest Beutler
Jan 5, 2001·Transplant International : Official Journal of the European Society for Organ Transplantation·U PozzettoF Serino
Dec 9, 2000·Transplant International : Official Journal of the European Society for Organ Transplantation·U PozzettoF Serino
Jun 7, 2007·Journal of Biomaterials Science. Polymer Edition·D LiH Yu
Apr 6, 2007·The AAPS Journal·Molly E Martin, Kevin G Rice
Feb 1, 2002·Biochemical and Biophysical Research Communications·Laurence VaysseBenoît Arveiler
May 5, 2007·Expert Opinion on Biological Therapy·Stefanos TheoharisPeng H Tan
Apr 29, 2008·Biochimica Et Biophysica Acta·Eric VivèsAndré Pèlegrin
Nov 27, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·R A Smith, T D Giorgio
May 1, 2013·Small·Huayu TianXuesi Chen
Sep 26, 2013·International Journal of Pharmaceutics·Hamideh ParhizMohammad Ramezani
Mar 12, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alexander WengStephen L Hart
Mar 17, 2006·Biochimica Et Biophysica Acta·Antoine KichlerBurkhard Bechinger
Apr 24, 2002·Pulmonary Pharmacology & Therapeutics·J VadolasP A Ioannou
Jun 19, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mehdi RezaeeBizhan Malaekeh-Nikouei
Jul 23, 2005·The Journal of Gene Medicine·Alan L ParkerJohn W Fabre
Mar 6, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephanie M GrosseStephen L Hart

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.