Integrin αvβ3-associated DAAM1 is essential for collagen-induced invadopodia extension and cell haptotaxis in breast cancer cells

The Journal of Biological Chemistry
Ting YanYichao Zhu

Abstract

The formin protein dishevelled-associated activator of morphogenesis 1 (DAAM1) polymerizes straight actin filaments and mediates migration of cancer cells. However, how DAAM1 governs cell haptotaxis in response to collagen remains unexplored in breast cancer cells. We hypothesized that DAAM1 mediates invadopodia extension and cell haptotaxis in response to type IV collagen in association with integrin receptors. Using Boyden chamber membranes coated with type IV collagen, we show here that type IV collagen activates both DAAM1 and Ras homolog family member A (RHOA) and promotes haptotaxis of MDA-MB-231 and MDA-MB-453 breast cancer cells, a process abolished by treatment with the integrin αvβ3 inhibitor cyclo(-RGDfK). shRNA-mediated knockdown of DAAM1 or a dominant-negative DAAM1 mutation (N-DAAM1) significantly decreased collagen-induced RHOA activity and the assembly of stress fibers, invadopodia extension, and cell haptotaxis. Immunoprecipitation and pulldown assays revealed that integrin αvβ3 is associated with, but only indirectly binds to, the C-terminal DAD domain of DAAM1 in mammalian cells. Blockade of RHOA activation with a specific inhibitor (CCG-1423) or via a dominant-negative RHOA mutation (RHOA-N19) suppressed col...Continue Reading

References

Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Isabelle PécheurPhilippe Clézardin
Aug 23, 2007·Science's STKE : Signal Transduction Knowledge Environment·Yingchun WangRichard L Klemke
Dec 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wei LiuRaymond Habas
May 7, 2008·The Journal of Cell Biology·Yan-Qing MaEdward F Plow
Dec 8, 2009·Science·Richard O Hynes
Jan 28, 2010·Biochimica Et Biophysica Acta·André Schönichen, Matthias Geyer
Dec 7, 2010·European Journal of Cell Biology·Eugen Kerkhoff
Jun 24, 2011·Nature Reviews. Molecular Cell Biology·Danielle A Murphy, Sara A Courtneidge
Oct 31, 2014·Nature Reviews. Molecular Cell Biology·Guillaume Charras, Erik Sahai
Jan 13, 2015·Nature Reviews. Molecular Cell Biology·Richard O Hynes
Feb 24, 2015·Frontiers in Cell and Developmental Biology·Abitha Jacob, Rytis Prekeris
Sep 14, 2015·Current Opinion in Cell Biology·Anne J Ridley
Jan 23, 2016·Molecular Biology of the Cell·Aude Guillabert-GourguesCecile Duplàa
Feb 10, 2016·Journal of Cell Science·Cédric Zeltz, Donald Gullberg
May 4, 2016·Cancer Discovery·Miguel Santiago-Medina, Jing Yang
Jul 22, 2016·Journal of Cell Science·Cédric Zeltz, Donald Gullberg
Jul 31, 2016·Current Opinion in Cell Biology·Fevzi Demircioglu, Kairbaan Hodivala-Dilke
Aug 26, 2016·Molecular Biology of the Cell·Madeleine J OudinFrank B Gertler
Nov 4, 2016·The Journal of Cell Biology·Tamako NishimuraMasatoshi Takeichi
Jan 5, 2017·Hormones & Cancer·Marina Inés FlaminiAngel Matías Sanchez
Jul 13, 2017·Cancers·Kristin A Kwakwa, Julie A Sterling

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Citations

May 6, 2019·Cancers·Rafael PeláezIgnacio M Larrayoz
Sep 16, 2019·Journal of Translational Medicine·Shuaishuai XuLiang Liu
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Ilenia MasiLaura Rosanò
Dec 27, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jie MeiYan Zhang
Feb 6, 2021·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Chenyang QiAgnes B Fogo
May 19, 2021·Cellular Signalling·Yasaswi Gayatri Mishra, Bramanandam Manavathi
Aug 29, 2021·Cell Death Discovery·Jie MeiYichao Zhu
Oct 19, 2020·European Journal of Cell Biology·Justine MaurinGuillaume Bompard

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