Integrins are required for cardioblast polarisation in Drosophila.

BMC Developmental Biology
Jessica VanderploegJ Roger Jacobs

Abstract

The formation of a tubular organ, such as the heart, requires the communication of positional and polarity signals between migratory cells. Key to this process is the establishment of a new luminal domain on the cell surface, generally from the apical domain of a migratory cell. This domain will also acquire basal properties, as it will produce a luminal extracellular matrix. Integrin receptors are the primary means of cell adhesion and adhesion signaling with the extracellular matrix. Here we characterise the requirement of Integrins in a genetic model of vasculogenesis, the formation of the heart in Drosophila. As with vertebrates, the Drosophila heart arises from lateral mesoderm that migrates medially to meet their contralateral partners, to then assemble a midline vessel. During migration, Integrins are among the first proteins restricted to the presumptive luminal domain of cardioblasts. Integrins are required for normal levels of leading edge membrane motility. Apical accumulation of Integrins is enhanced by Robo, and reciprocally, apicalisation of luminal factors like Slit and Robo requires Integrin function. Integrins may provide a template for the formation of a lumen by stabilising lumen factors like Robo. Subsequent...Continue Reading

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Citations

Aug 4, 2015·Journal of Cardiovascular Development and Disease·Georg Vogler, Rolf Bodmer
Jan 18, 2015·Developmental Biology·Aidan P Maartens, Nicholas H Brown
May 11, 2015·Developmental Biology·Jessica Vanderploeg, J Roger Jacobs
Apr 15, 2014·Methods : a Companion to Methods in Enzymology·Karen OcorrRolf Bodmer
Jun 10, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shaad M Ahmad
Apr 12, 2016·Journal of Cardiovascular Development and Disease·Barbara Rotstein, Achim Paululat
Nov 30, 2021·Biology Open·Mariana Barrera-Velázquez, Luis Daniel Ríos-Barrera

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Methods Mentioned

BETA
GTPases
dissection
PCR
confocal microscopy

Software Mentioned

OpenLab
Adobe Photoshop
ImageJ
Pericardin

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