Integrins Form an Expanding Diffusional Barrier that Coordinates Phagocytosis

Cell
Spencer A FreemanSergio Grinstein

Abstract

Phagocytosis is initiated by lateral clustering of receptors, which in turn activates Src-family kinases (SFKs). Activation of SFKs requires depletion of tyrosine phosphatases from the area of particle engagement. We investigated how the major phosphatase CD45 is excluded from contact sites, using single-molecule tracking. The mobility of CD45 increased markedly upon engagement of Fcγ receptors. While individual CD45 molecules moved randomly, they were displaced from the advancing phagocytic cup by an expanding diffusional barrier. By micropatterning IgG, the ligand of Fcγ receptors, we found that the barrier extended well beyond the perimeter of the receptor-ligand engagement zone. Second messengers generated by Fcγ receptors activated integrins, which formed an actin-tethered diffusion barrier that excluded CD45. The expanding integrin wave facilitates the zippering of Fcγ receptors onto the target and integrates the information from sparse receptor-ligand complexes, coordinating the progression and ultimate closure of the phagocytic cup.

References

Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·H KawasakiA M Graybiel
Jan 9, 1999·The Journal of Biological Chemistry·D CoxS Greenberg
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K G JohnsonM L Thomas
Jul 21, 2006·Nature Immunology·Simon J Davis, P Anton van der Merwe
Apr 21, 2007·International Immunology·Karel DrbalGerhard J Schütz
May 12, 2007·The Journal of Clinical Investigation·Wolfgang BergmeierDenisa D Wagner
Jul 22, 2008·Nature Methods·Khuloud JaqamanGaudenz Danuser
Nov 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alexis J TorresBarbara A Baird
Aug 20, 2009·Journal of Cell Science·Sei YoshidaJoel A Swanson
Jan 6, 2010·The Journal of Cell Biology·Feng YeMark H Ginsberg
Feb 19, 2010·The Journal of Biological Chemistry·Christopher W CairoDavid E Golan
Dec 1, 2010·The Journal of Cell Biology·Michal BohdanowiczSergio Grinstein
Aug 9, 2011·Trends in Cell Biology·Martijn Gloerich, Johannes L Bos
Sep 14, 2011·Annual Review of Pathology·Ronald S FlannaganSergio Grinstein

❮ Previous
Next ❯

Citations

Mar 18, 2016·Immunity·Siamon Gordon
Aug 26, 2016·Immunological Reviews·Roni LevinJohnathan Canton
Sep 27, 2016·Current Opinion in Cell Biology·Jesse Goyette, Katharina Gaus
Aug 9, 2016·Annual Review of Cell and Developmental Biology·Michael L Dustin, Kaushik Choudhuri
Oct 12, 2016·Scientific Reports·Maria GoikoBryan Heit
Oct 21, 2016·Médecine sciences : M/S·Coralie AlexandreOliver Nüsse
Nov 5, 2016·The Journal of Cell Biology·Merja JoensuuFrédéric A Meunier
May 26, 2017·The FEBS Journal·Catherine M Buckley, Jason S King
Apr 14, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kirstie L S ClearyMark S Cragg
Dec 20, 2017·Frontiers in Immunology·Nicole D BarthIan Dransfield
Jun 5, 2018·ELife·Meghan A MorrisseyRonald D Vale
Aug 10, 2016·Journal of Cell Science·Nele M G DieckmannGillian M Griffiths
Sep 14, 2018·Journal of Cell Science·Edward JenkinsErdinc Sezgin
Jan 3, 2019·Endocrinology·Anne R Bresnick, Jonathan M Backer
Aug 2, 2017·Nature Reviews. Immunology·Morgan Huse
Dec 1, 2017·Cell and Tissue Research·Charles Yin, Bryan Heit
Dec 27, 2019·Molecular Biology of the Cell·Marcos Francisco NúñezSarah L Veatch
Jul 16, 2020·American Journal of Physiology. Cell Physiology·János G Filep, Amiram Ariel
Jul 12, 2017·BioMed Research International·Carlos Rosales, Eileen Uribe-Querol
Mar 29, 2018·International Journal of Molecular Sciences·Jiro TakitoMasanori Nakamura
Jul 14, 2018·Nature Reviews. Cancer·Hellyeh Hamidi, Johanna Ivaska
Sep 14, 2018·Seminars in Immunopathology·Sivakami M MylvaganamSpencer A Freeman
Feb 9, 2019·Frontiers in Chemistry·Marco LindnerEva Sevcsik
Aug 29, 2019·Frontiers in Pharmacology·Simone ArientiIan Dransfield
May 20, 2020·Frontiers in Immunology·Alvaro Torres-GomezEsther M Lafuente
Jun 21, 2020·Nature Protocols·Aaron M JoffeDaniel A Fletcher
Jun 5, 2020·F1000Research·Glenn F W Walpole, Sergio Grinstein
Mar 16, 2017·The Journal of Cell Biology·Filipa B LopesDaniel M Davis
Mar 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Stacy A MalakerCarolyn R Bertozzi
Feb 13, 2019·Journal of Cell Science·Jesse GoyetteKatharina Gaus
Jun 30, 2019·Nature Communications·Anita KumariPaolo Pierobon
Jul 1, 2020·Frontiers in Immunology·Valentin Jaumouillé, Clare M Waterman
Feb 12, 2017·Pflügers Archiv : European journal of physiology·Siamon Gordon, Luisa Martinez-Pomares
Aug 25, 2018·The Journal of Cell Biology·Adam P Williamson, Ronald D Vale
Mar 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Meriem SekheriJános G Filep
Sep 15, 2017·Engineering in Life Sciences·Cindy Dirscherl, Sebastian Springer
Jun 26, 2020·Frontiers in Immunology·Eileen Uribe-Querol, Carlos Rosales
Jul 29, 2019·The Journal of Membrane Biology·Laurent Limozin, Pierre-Henri Puech
Dec 17, 2020·Journal for Immunotherapy of Cancer·Alan Herbert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.