Integrins Influence the Size and Dynamics of Signaling Microclusters in a Pyk2-dependent Manner.

The Journal of Biological Chemistry
Maria SteblyankoYuri Sykulev

Abstract

Integrin engagement on lymphocytes initiates "outside-in" signaling that is required for cytoskeleton remodeling and the formation of the synaptic interface. However, the mechanism by which the "outside-in" signal contributes to receptor-mediated intracellular signaling that regulates the kinetics of granule delivery and efficiency of cytolytic activity is not well understood. We have found that variations in ICAM-1 expression on tumor cells influence killing kinetics of these cells by CD16.NK-92 cytolytic effectors suggesting that changes in integrin ligation on the effector cells regulate the kinetics of cytolytic activity by the effector cells. To understand how variations of the integrin receptor ligation may alter cytolytic activity of CD16.NK-92 cells, we analyzed molecular events at the contact area of these cells exposed to planar lipid bilayers that display integrin ligands at different densities and activating CD16-specific antibodies. Changes in the extent of integrin ligation on CD16.NK-92 cells at the cell/bilayer interface revealed that the integrin signal influences the size and the dynamics of activating receptor microclusters in a Pyk2-dependent manner. Integrin-mediated changes of the intracellular signaling s...Continue Reading

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Citations

Dec 7, 2018·Frontiers in Immunology·Jérémie RossyDaniel F Legler
Mar 7, 2019·Frontiers in Immunology·Kashyap R PatelAdam W Barb
Jul 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Samuel M S Cheung, Hanne L Ostergaard
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Dec 14, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nadia AnikeevaYuri Sykulev
Feb 13, 2021·Cancers·Pinelopi A NikolopoulouVassiliki Kostourou
Feb 12, 2021·Bone Research·Ji-Won LeeSek Won Kong
Mar 9, 2021·Frontiers in Immunology·Panagiota BoutiTaco W Kuijpers
Aug 3, 2021·Frontiers in Immunology·Giorgio SantoniAngela Santoni
Aug 10, 2021·The Journal of Biological Chemistry·Nadia AnikeevaYuri Sykulev

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