Oct 25, 2018

Integrity and stability of virulence plasmids from Pseudomonas syringae are modulated by mobile genetic elements and multiple toxin-antitoxin systems

BioRxiv : the Preprint Server for Biology
Leire BardajiJ Murillo

Abstract

Background: Virulence plasmids are critically exposed to genetic decay and loss, particularly in Pseudomonas syringae strains because of their high content of mobile genetic elements and their exploitation of environmental niches outside of the plant host. The demonstrated high plasticity and adaptability of P. syringae plasmids, involving the acquisition and loss of large DNA regions, contrasts with their usual high stability and the maintenance of key virulence genes in free living conditions. The identification of plasmid stability determinants and mechanisms will help to understand their evolution and adaptability to agroecosystems as well as to develop more efficient control measures. Results: We show that the three virulence plasmids of P. syringae pv. savastanoi NCPPB 3335 contain diverse functional stability determinants, including three toxin-antitoxin systems (TA) in both pPsv48A and pPsv48C, whereas one of the two replicons of pPsv48C can confer stable inheritance by itself. Loss of pPsv48A increased by two orders of magnitude upon functional inactivation of its TA systems. However, inactivation of the TA systems from pPsv48C did not result in its curing but led to the recovery of diverse deletion derivatives. One ty...Continue Reading

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Mentioned in this Paper

In Vivo
Derivatives
Genes
Transposition of Intestine (Disorder)
Toxin
Phytophthora syringae
Environment
Virus Replication
Recombination, Genetic
Gene Deletion Abnormality

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