Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Martin ThomassenJ Bangsbo

Abstract

The present study examined the effect of intensive training in combination with marked reduction in training volume on phospholemman (FXYD1) expression and phosphorylation at rest and during exercise. Eight well-trained cyclists replaced their regular training with speed-endurance training (10-12 × ∼30-s sprints) two or three times per week and aerobic high-intensity training (4-5 × 3-4 min at 90-95% of peak aerobic power output) 1-2 times per week for 7 wk and reduced the training volume by 70%. Muscle biopsies were obtained before and during a repeated high-intensity exercise protocol, and protein expression and phosphorylation were determined by Western blot analysis. Expression of FXYD1 (30%), actin (40%), mammalian target of rapamycin (mTOR) (12%), phospholamban (PLN) (16%), and Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) γ/δ (25%) was higher (P < 0.05) than before the training intervention. In addition, after the intervention, nonspecific FXYD1 phosphorylation was higher (P < 0.05) at rest and during exercise, mainly achieved by an increased FXYD1 Ser-68 phosphorylation, compared with before the intervention. CaMKII, Thr-287, and eukaryotic elongation factor 2 Thr-56 phosphorylation at rest and during exercise,...Continue Reading

References

Feb 9, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C JuelJ Bangsbo
Mar 9, 2002·The Journal of Biological Chemistry·Thomas H ReynoldsJohn C Lawrence
Aug 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gilles CrambertKathi Geering
Dec 19, 2002·American Journal of Physiology. Endocrinology and Metabolism·Jorgen F P WojtaszewskiErik A Richter
Sep 25, 2003·Physiological Reviews·Torben Clausen
Nov 14, 2003·American Journal of Physiology. Endocrinology and Metabolism·Christian FrøsigJørgen F P Wojtaszewski
Sep 24, 2004·American Journal of Physiology. Endocrinology and Metabolism·Carsten RoepstorffBente Kiens
Dec 29, 2004·Cardiovascular Research·Benjamin d Z SilvermanMichael J Shattock
Sep 13, 2005·Journal of Vascular Research·Christopher M RemboldJ Randall Moorman
Nov 4, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vernon G CoffeyJohn A Hawley
Feb 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Davor PavlovićMichael J Shattock
Jul 27, 2007·Cell Biochemistry and Biophysics·William W Winder, David M Thomson
Oct 27, 2007·Journal of Applied Physiology·Michael J McKennaJean-Marc Renaud
Nov 10, 2007·The Journal of Biological Chemistry·Stéphanie BibertKäthi Geering
Dec 21, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·F Marcello IaiaJens Bangsbo
Jan 16, 2008·Physiological Reviews·D G AllenH Westerblad
Oct 2, 2008·American Journal of Physiology. Endocrinology and Metabolism·Boubacar BenzianeNigel K Stepto
Apr 3, 2009·American Journal of Physiology. Cell Physiology·William FullerMichael J Shattock
Dec 7, 2010·European Journal of Applied Physiology·K Van ProeyenP Hespel
Dec 18, 2010·European Journal of Applied Physiology·Vernon G CoffeyJohn A Hawley
Feb 18, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martin Krøyer RasmussenNikolai B Nordsborg
Jun 10, 2011·American Journal of Physiology. Endocrinology and Metabolism·Boubacar BenzianeAlexander V Chibalin
Jun 21, 2011·The Journal of Membrane Biology·Maria S IngwersenCarsten Juel
Aug 19, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ali El-ArmoucheDavor Pavlovic
Oct 1, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martin ThomassenNikolai B Nordsborg
Jan 30, 2013·The Journal of Physiology·Martin ThomassenJens Bangsbo
Apr 25, 2013·Journal of Molecular and Cellular Cardiology·Davor PavlovicMichael J Shattock
May 2, 2013·American Journal of Physiology. Endocrinology and Metabolism·William ApróKent Sahlin
May 16, 2013·Journal of Molecular and Cellular Cardiology·Davor PavlovicMichael J Shattock
Jul 26, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Thomas P GunnarssonJens Bangsbo

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Citations

Aug 18, 2016·Frontiers in Physiology·Victoria L Wyckelsma, Michael J McKenna
May 12, 2016·American Journal of Physiology. Endocrinology and Metabolism·Sergej Pirkmajer, Alexander V Chibalin
Sep 20, 2020·Scandinavian Journal of Medicine & Science in Sports·Miriam Martinez-CantonJose A L Calbet
Jan 6, 2021·Journal of Muscle Research and Cell Motility·Sergej PirkmajerAlexander V Chibalin
Jun 2, 2021·Journal of Cachexia, Sarcopenia and Muscle·James E M BlackwellBethan E Phillips

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