Inter-generational Consequences for Growing C. elegans in Liquid

BioRxiv : the Preprint Server for Biology
Itamar LevOded Rechavi

Abstract

In recent years, studies in Caenorhabditis elegans nematodes have shown that different stresses can generate multigenerational changes. Here we show that worms that grow in liquid media, and also their plate-grown progeny, are different from worms whose ancestors were grown on plates. It has been suggested that C. elegans might encounter liquid environments in nature, although actual observations in the wild are few and far between. In contrast, in the lab, growing worms in liquid is commonplace, and often used as an alternative to growing worms on agar plates, to control the composition of the worms' diet, to starve (and synchronize) worms, or to grow large populations for biochemical assays. We found that plate-grown descendants of M9 liquid media-grown worms were longer than control worms, and the heritable effects were apparent already very early in development. We tested for the involvement of different known epigenetic inheritance mechanisms, but could not find a single mutant in which these inter-generational effects are canceled. While we found that growing in liquid always leads to inter-generational changes in the worms' size, trans-generational effects were found to be variable, and in some cases the effects were gon...Continue Reading

Related Concepts

Diet
Environment
Laboratory
Physiological Aspects
Caenorhabditis elegans
Size
Mutant
MORG1 protein, mouse
Epigenetic Process
Study

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