Abstract
Genetic recombination plays a significant role in the survival and evolution of hepatitis C virus (HCV), but methodological limitations have hindered the exploration of genetic recombination. HCV serotypes were evaluated in 104 patients with chronic hepatitis C when they initially presented in hospitals. Subsequently, HCV genotypes were analyzed using primers for core gene and NS5B gene. Near-complete nucleotide sequences of eight HCV isolates from two suspected patients with 2b/1b recombinant HCV were analyzed by amplification of nine overlapping regions of HCV-specific oligonucleotide primers at different time points: (i) at the first admission; (ii) before and (iii) after interferon therapy; and (iv) after development of hepatocellular carcinoma. The nucleotide sequence of eight HCV isolates obtained was 9,321-9,471 nucleotides in length, comprising a single ORF (polyprotein of 3,014 amino acids.) and segregated into discordant genotypes of 2b and 1b HCV with a recombination junction in NS2. This study highlights the need for more precise characterization of HCV in clinical samples where there is a discrepancy between immunoassays and sequencing. It also demonstrates the circulation of novel inter-genotypic recombinant HCV i...Continue Reading
References
Oct 1, 1992·Virology·H OkamotoS Mishiro
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooP J Barr
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N OgataR H Purcell
Jan 11, 1996·The New England Journal of Medicine·N EnomotoC Sato
Nov 1, 1995·Journal of Clinical Microbiology·V DixitJ Kolberg
Oct 2, 1998·Science·A U NeumannA S Perelson
May 20, 1999·Molecular Biology and Evolution·E C HolmesA Rambaut
Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Patrizia FarciRobert H Purcell
Mar 22, 2002·Journal of Virology·Olga KalininaLars O Magnius
Oct 31, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Adrian M Di Bisceglie, Jay H Hoofnagle
Jan 14, 2004·The Journal of General Virology·Rodney ColinaJuan Cristina
Feb 15, 2005·Journal of Virology·Thomas J ChambersAdrian M Dibisceglie
Sep 26, 2006·Journal of Medical Virology·Seiji KageyamaHiroshi Ichimura
Dec 16, 2006·The Journal of General Virology·Fengxiang GaoHarold S Margolis
May 10, 2007·Nature Reviews. Microbiology·Darius MoradpourCharles M Rice
Oct 18, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Haruaki ShirakawaUNKNOWN Nagano Interferon Treatment Research Group
Mar 31, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marc G GhanyUNKNOWN American Association for the Study of Liver Diseases
May 1, 2009·Emerging Infectious Diseases·Kazuaki TakahashiShunji Mishiro
Jan 14, 2010·Intervirology·Hobyung ChungMasatoshi Kudo
Jan 18, 2011·Journal of Viral Hepatitis·V MorelS Castelain
Jun 7, 2011·Archives of Virology·Koji YokoyamaMariko Y Momoi
Citations
Mar 19, 2014·Trends in Microbiology·Andrea Galli, Jens Bukh
Oct 8, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Virginie MorelSandrine Castelain
Oct 12, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Donald B SmithPeter Simmonds
Jul 31, 2013·Virology·Laura Esteban-RiescoCatherine Gaudy-Graffin
Jul 24, 2015·Journal of Clinical Microbiology·James C IlesOliver G Pybus
May 6, 2015·World Journal of Hepatology·Natalia EcheverríaPilar Moreno
Jan 5, 2017·The Tohoku Journal of Experimental Medicine·Jun InoueTooru Shimosegawa
Sep 6, 2017·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Hayato KurataSatoshi Mochida
Nov 18, 2018·Clinical Journal of Gastroenterology·Masako OkadaNorifumi Kawada
Apr 27, 2021·Internal Medicine·Masami MinemuraIchiro Yasuda