Inter-individual differences in HLA expression can impact the CDC crossmatch

Tissue Antigens
G HöngerC Hess

Abstract

How human leucocyte antigen (HLA) expression levels on human lymphocytes relate to clinically relevant in vitro cytotoxicity testing has not been defined. Here, cross-sectional (n = 14) and longitudinal (n = 6) semi-quantitative assessment of HLA expression on lymphocytes was performed. Complement-dependent cytotoxicity (CDC) and cellular allo-reactivity were assessed vis-à-vis target cells with defined levels of HLA expression. On CD4(+) and CD8(+) T-cells, and on B-cells, intra-individual HLA levels varied ≤1.5-fold, whereas inter-individual HLA expression varied 2.34-fold and 2.07-fold on CD4(+) and CD8(+) T-cells, respectively, and 2.90-fold on B-cells. Importantly, CDC crossmatch reactions induced by anti-HLA-A2 monoclonal antibody as well as patient sera solely containing HLA-A2 antibodies were significantly impacted by HLA-A2 expression levels on donor cells. Likewise, cytotoxicity of HLA-A2 reactive effector cells was induced proportionate to availability of HLA-A2. These data demonstrate that human HLA expression on lymphocytes from healthy blood donors is fairly stable intra-individually, yet varies significantly from person to person. Variability in HLA expression levels can impact functional cytotoxic reactions in v...Continue Reading

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Citations

Sep 25, 2015·European Journal of Immunology·Céline RenéJean-François Eliaou
Aug 18, 2016·Transplantation Reviews·Antonietta PicasciaClaudio Napoli
Mar 3, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M WahrmannG A Böhmig
Apr 1, 2017·Transplantation Direct·Caroline WehmeierStefan Schaub
Feb 22, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·E G KamburovaH G Otten
Jul 31, 2019·Transplant International : Official Journal of the European Society for Organ Transplantation·Caroline WehmeierStefan Schaub
Jan 26, 2020·HLA : Immune Response Genetics·B Sean CareyAnthony Poles
Sep 8, 2017·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Sarah AbbesOluwatoyosi Onwuemene
Jun 7, 2017·HLA : Immune Response Genetics·G HöngerS Schaub
Jun 12, 2019·International Journal of Immunogenetics·B Sean CareyAnthony Poles
Oct 15, 2020·HLA : Immune Response Genetics·Jonathan VisentinJean-Luc Taupin

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