Inter-kingdom effect on epithelial cells of the N-Acyl homoserine lactone 3-oxo-C12:2, a major quorum-sensing molecule from gut microbiota

PloS One
Cecilia LandmanSaint Antoine IBD Network

Abstract

N-acyl homoserine lactones (AHLs), which are autoinducer quorum-sensing molecules involved in the bacterial communication network, also interact with eukaryotic cells. Searching for these molecules in the context of inflammatory bowel disease (IBD) is appealing. The aims of our study were to look for AHL molecules in faecal samples from healthy subjects (HS) and IBD patients to correlate AHL profiles with the microbiome and investigate the effect of AHLs of interest on epithelial cells. Using mass spectrometry, we characterised AHL profiles in faecal samples from HS (n = 26) and IBD patients in remission (n = 24) and in flare (n = 25) and correlated the presence of AHLs of interest with gut microbiota composition obtained by real-time qPCR and 16S sequencing. We synthesised AHLs of interest to test the inflammatory response after IL1β stimulation and paracellular permeability on Caco-2 cells. We observed 14 different AHLs, among which one was prominent. This AHL corresponded to 3-oxo-C12:2 and was found significantly less frequently in IBD patients in flare (16%) and in remission (37.5%) versus HS (65.4%) (p = 0.001). The presence of 3-oxo-C12:2 was associated with significantly higher counts of Firmicutes, especially Faecalbac...Continue Reading

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Citations

Aug 1, 2020·The American Journal of Psychiatry·Eva M Medina-RodriguezEléonore Beurel
Sep 29, 2020·Frontiers in Immunology·Garance CoquantPhilippe Seksik
Jun 26, 2020·Frontiers in Microbiology·Xingyan MaYang Lu
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Jan 15, 2021·Microbial Pathogenesis·Leonardo Luiz de FreitasMaria Cristina Dantas Vanetti
Dec 17, 2020·International Journal of Molecular Sciences·Agathe PeyrottesJean-Maurice Mallet
Feb 16, 2021·Frontiers in Microbiology·Liang Wu, Yubin Luo
Jun 30, 2021·Biochimie·Kristyna Krasulova, Peter Illes

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Methods Mentioned

BETA
biosensor
FCS
enzyme-linked immunosorbent assay
16S sequencing

Software Mentioned

Jump
BLAST
LEfSe
Permanova
COBALT
000
JMP SAS
MaAsLin )
GraphPad Prism
Analyst®

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