Inter-rater reliability of malaria parasite counts and comparison of methods.

Malaria Journal
Katherine M BowersHilary Watt

Abstract

The introduction of artemesinin-based treatment for falciparum malaria has led to a shift away from symptom-based diagnosis. Diagnosis may be achieved by using rapid non-microscopic diagnostic tests (RDTs), of which there are many available. Light microscopy, however, has a central role in parasite identification and quantification and remains the main method of parasite-based diagnosis in clinic and hospital settings and is necessary for monitoring the accuracy of RDTs. The World Health Organization has prepared a proficiency testing panel containing a range of malaria-positive blood samples of known parasitaemia, to be used for the assessment of commercially available malaria RDTs. Different blood film and counting methods may be used for this purpose, which raises questions regarding accuracy and reproducibility. A comparison was made of the established methods for parasitaemia estimation to determine which would give the least inter-rater and inter-method variation Experienced malaria microscopists counted asexual parasitaemia on different slides using three methods; the thin film method using the total erythrocyte count, the thick film method using the total white cell count and the Earle and Perez method. All the slides w...Continue Reading

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Citations

Sep 24, 2011·Malaria Journal·N J White
Sep 19, 2012·Malaria Journal·Helena H AsklingUNKNOWN European Society for Clinical Microbiology and Infectious Diseases Study Group on Clinical Parasitology
Sep 24, 2013·Malaria Journal·Mounkaila Abdou BilloOgobara K Doumbo
Nov 14, 2013·British Journal of Haematology·J Wendi BaileyUNKNOWN General Haematology Task Force of the British Committee for Standards in Haematology
Jun 13, 2014·International Journal of Language & Communication Disorders·Ana Rita S ValenteMargaret Leahy
Mar 8, 2013·Travel Medicine and Infectious Disease·Tomas Jelinek
Jul 28, 2016·PLoS Neglected Tropical Diseases·Kathryn Shaw-SalibaErica M Pasini
Nov 28, 2019·Malaria Journal·Billy Ngasala, Samweli Bushukatale
Sep 6, 2020·Malaria Journal·Mehul DhordaUNKNOWN Research Malaria Microscopy Working Group
Aug 18, 2016·Expert Review of Anti-infective Therapy·Brian T Grimberg, Kerry O Grimberg
Dec 15, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T GrossmanE Marva
Apr 14, 2020·American Journal of Hematology·Petru ManescuDelmiro Fernandez-Reyes

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Methods Mentioned

BETA
light microscopy
PCR

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