Inter-relationship between immunoglobulin idiotype and metatype.

Molecular Immunology
E W VossD W Ballard

Abstract

Allogenic anti-metatype (Met) and anti-idiotype (Id) reagents were elicited to the liganded and nonliganded states, respectively, of a high affinity murine monoclonal anti-fluorescein IgM antibody. Through comparisons of the relative immunogenicity and specificity patterns of the resulting antibody reagents, interpretations regarding the relationship between metatopes and idiotopes were rendered. Anti-Id specificity was measured in terms of the degree of ligand inhibition to two different forms of the fluorescyl hapten (i.e. free ligand and conjugated to a macromolecule). Idiotypic analysis of 18-2-3 H and L chains (immunoglobulin heavy and light chains) demonstrated that recognition of 18-2-3 Id determinants required recombination of H and L chains. Anti-Met reagents were evaluated relative to the liganded and nonliganded states of the IgM antibody. Binding studies indicated anti-Met specificity for liganded or affinity labeled Mab (monoclonal antibody) 18-2-3, but not for nonliganded 18-2-3 or the fluorescein ligand. The affinity labeled metatypic state provided the optimum immunogen yielding an antibody reagent which was rendered specific for the liganded state upon absorption with appropriate immunoglobulin reagents. Antibo...Continue Reading

References

May 1, 1976·Immunochemistry·E W VossR T Root
Jan 1, 1986·Annual Review of Immunology·J M DavieB L Clevinger
Apr 1, 1988·Revue française de transfusion et immuno-hématologie·P Tippett, C A Green
Dec 1, 1988·Molecular Immunology·M A Dombrink-Kurtzman, E W Voss
Mar 1, 1988·Immunological Investigations·E W VossS D Miklasz
Jan 1, 1984·Annual Review of Immunology·D C BenjaminE M Prager
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D W BallardE W Voss
Jan 1, 1983·Annual Review of Immunology·D R Davies, H Metzger
Nov 1, 1983·Journal of Biochemical and Biophysical Methods·J N Herron, E W Voss
Jan 1, 1985·Immunology Today·B F Erlanger

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Citations

Oct 4, 1990·Journal of Immunological Methods·D Samuel, R A Abuknesha
Feb 11, 1992·Molecular Immunology·E W VossV H Voss
May 13, 2010·Analytical Chemistry·Norihiro KobayashiJunichi Goto

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