Inter-specific analysis of Drosophila alcohol dehydrogenase by an immunoenzymatic assay using monoclonal antibodies

Biochemical and Biophysical Research Communications
J FiblaR Gonzàlez-Duarte

Abstract

Three Drosophila alcohol dehydrogenase monoclonal antibodies have been prepared and characterized. These antibodies cross-react with alcohol dehydrogenase from different species as revealed by immunoblotting assay. An enzyme-linked immunosorbent assay has been devised to quantify alcohol dehydrogenase in several species, different strains and individual larval organs. The assay detects alcohol dehydrogenase via a double-antibody sandwich assay technique giving strictly proportional values for antigen concentration and optical densities in the range of 3-30 ng of antigen per 100 microliters of sample. When alcohol dehydrogenase specific activity is compared in different larval organs a remarkable similarity is observed, whereas protein distribution varies substantially. Larval fat body and larval alimentary canal contribute 63% and 26% respectively to recovered alcohol dehydrogenase.

Citations

Feb 1, 1993·Journal of Biochemical and Biophysical Methods·J Fibla, R Gonzàlez-Duarte
Aug 6, 1991·Biochimica Et Biophysica Acta·Y W HoW K Ho
Jun 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B W GeerS W McKechnie
Apr 21, 1999·Biochimica Et Biophysica Acta·M Kaaber BrendskagJ O Winberg
Jan 15, 1993·European Journal of Biochemistry·J FiblaR Gonzàlez-Duarte
Sep 4, 2004·Genome Biology·Jing WangJulian A T Dow

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