Interacting factors that influence long-term storage of live Pasteurella tularensis vaccine and Rift Valley fever virus.

Applied Microbiology
F KleinR E Lincoln


Studies were conducted on the interaction of various parameters which affect the storage stability and growth potential of liquid cultures of Pasteurella tularensis live vaccine strain (LVS) and Rift Valley fever virus Van Wyk strain (RVFV). Storage variables studied with LVS included four storage temperatures (4, -20, -65, -175 C), single and multiple freeze-thaw cycles, two freezing and two thawing rates (slow and fast), various inoculum levels (1, 3, 5, and 10%) for the determination of growth potential, and the retention of immunizing potential (mice and guinea pig) after storage. Neither the freezing rate nor the number of freeze-thaw cycles seriously affected the growth of LVS after storage at -175C; however, the slow rate of thaw proved deleterious as were all temperatures of storage except -175 C after 1 year of storage, as shown by both criteria of evaluation. RVFV produced in two combinations of cell lines and media (LM cell line-199 peptone medium and LDR cell line-Eagle's minimum essential medium) was stored at three serum levels (10, 20, 40%), three pH values (6.2., 7.0, 7.8), and three temperatures (-20, -65, -175 C). These studies indicated: (i) virus produced in the LDR cell line and Eagle's medium was more stab...Continue Reading


May 1, 1967·Cryobiology·W A Rightsel, D Greiff
Jun 1, 1968·Applied Microbiology·F J WeiretherR E Lincoln
Jun 1, 1968·Applied Microbiology·J M ScharerR E Lincoln
May 1, 1966·Cryobiology·D Greiff, R T Kelly
Aug 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·R J KUCHLER, D J MERCHANT
Jan 1, 1947·Journal of Bacteriology·C M DOWNS, L L CORIELL

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