Interacting molecule of AT1 receptor, ATRAP, is colocalized with AT1 receptor in the mouse renal tubules

Kidney International
Yuko TsurumiSatoshi Umemura

Abstract

The renin-angiotensin system in the kidney plays a critical role in the regulation of renal hemodynamics and sodium handling through the activation of vascular, glomerular and tubular angiotensin II type 1 (AT1) receptor-mediated signaling. We previously cloned a molecule that specifically bound to the AT1 receptor and modulated AT1 receptor signaling in vitro, which we named ATRAP (for AT1 receptor-associated protein). The purpose of this study is to analyze the renal distribution of ATRAP and to examine whether ATRAP is co-expressed with the AT1 receptor in the mouse kidney. We performed in situ hybridization, Western blot analysis, and immunohistochemistry to investigate the expression of ATRAP mRNA and protein in the mouse kidney. The results of Western blot analysis revealed the ATRAP protein to be abundantly expressed in the kidney. Employing in situ hybridization and immunohistochemistry, we found that both ATRAP mRNA and the protein were widely distributed along the renal tubules from Bowman's capsules to the inner medullary collecting ducts. ATRAP mRNA was also detected in the glomeruli, vasculature, and interstitial cells. In all tubular cells, the ATRAP protein colocalized with the AT1 receptor. Finally, we found tha...Continue Reading

References

Jan 1, 1986·The Journal of Clinical Investigation·S K MujaisA I Katz
Mar 1, 1993·Journal of the American Society of Nephrology : JASN·G Wolf, E G Neilson
Jun 20, 1996·The New England Journal of Medicine·T L GoodfriendK J Catt
Feb 22, 2001·Journal of the American Society of Nephrology : JASN·Hiroyuki KoboriL Gabriel Navar
Apr 19, 2002·Journal of the American Society of Nephrology : JASN·János Peti-PeterdiP Darwin Bell
Aug 23, 2002·Journal of the American Society of Nephrology : JASN·Akira NishiyamaL Gabriel Navar
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Catherine IngertJean-Louis Imbs
Dec 6, 2003·The EMBO Journal·Takaaki SenbonmatsuTadashi Inagami

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Citations

May 17, 2012·Pflügers Archiv : European journal of physiology·Hayo Castrop
Apr 20, 2007·Current Hypertension Reports·Kouichi TamuraMiyuki Matsuda
May 9, 2013·The Journal of Biological Chemistry·Miyuki MatsudaSatoshi Umemura
May 12, 2009·Current Opinion in Nephrology and Hypertension·Masaki MogiMasatsugu Horiuchi
Jan 23, 2010·Journal of the American Society of Nephrology : JASN·Mona OppermannHayo Castrop
May 17, 2014·International Journal of Medical Sciences·Akinobu MaedaSatoshi Umemura
Sep 23, 2008·Pharmacology & Therapeutics·Emma S JonesRobert E Widdop
Jan 7, 2015·Current Opinion in Pharmacology·Hayo Castrop
May 3, 2012·Clinical Science·Masatsugu HoriuchiMasaki Mogi
Jun 7, 2018·Physiological Reviews·Steven J ForresterSatoru Eguchi
Mar 6, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Yuko Tsurumi-IkeyaSatoshi Umemura
Jul 29, 2017·Journal of the American Heart Association·Kazushi UnedaKouichi Tamura
Aug 2, 2013·Journal of the American Heart Association·Akinobu MaedaSatoshi Umemura
Aug 25, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Masaki MogiMasatsugu Horiuchi
Apr 13, 2019·Journal of the American Heart Association·Sho KinguchiKouichi Tamura
Aug 27, 2010·American Journal of Physiology. Renal Physiology·Hiromichi WakuiSatoshi Umemura
Apr 16, 2010·Physiological Reviews·Hayo CastropCharlotte Wagner
Feb 15, 2007·American Journal of Physiology. Renal Physiology·Masashi SakaiSatoshi Umemura
Feb 24, 2012·American Journal of Physiology. Renal Physiology·Elisabeth DoblingerHayo Castrop
Aug 6, 2010·American Journal of Physiology. Renal Physiology·Shin-ichiro MasudaSatoshi Umemura
Oct 14, 2021·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Kouichi TamuraTakahiro Yamaji

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