Interaction between Alzheimer's Abeta(25-35) peptide and phospholipid bilayers: the role of cholesterol

Biochimica Et Biophysica Acta
Gerardino D'ErricoAnna M D'Ursi

Abstract

There is mounting evidence that the lipid matrix of neuronal cell membranes plays an important role in the accumulation of beta-amyloid peptides into senile plaques, one of the hallmarks of Alzheimer's disease (AD). With the aim to clarify the molecular basis of the interaction between amyloid peptides and cellular membranes, we investigated the interaction between a cytotoxic fragment of Abeta(1-42), i.e., Abeta(25-35), and phospholipid bilayer membranes. These systems were studied by Electron Paramagnetic Resonance (EPR) spectroscopy, using phospholipids spin-labeled on the acyl chain. The effect of inclusion of charged phospholipids or/and cholesterol in the bilayer composition was considered in relation to the peptide/membrane interaction. The results show that Abeta(25-35) inserts in bilayers formed by the zwitterionic phospholipid dilauroyl phosphatidylcholine (DLPC), positioning between the outer part of the hydrophobic core and the external hydrophilic layer. This process is not significantly influenced by the inclusion of the anionic phospholipid phosphatidylglycerol (DLPG) in the bilayer, indicating the peptide insertion to be driven by hydrophobic rather than electrostatic interactions. Cholesterol plays a fundamenta...Continue Reading

References

Oct 3, 1999·European Journal of Biochemistry·M Del Mar Martínez-SenacJ C Gómez-Fernández
Feb 19, 2000·Current Opinion in Structural Biology·J C Rochet, P T Lansbury
Jul 20, 2002·Peptides·Meng-chin Allison Lin, Bruce L Kagan
Nov 5, 2003·Biochemistry·Muthu RamakrishnanDerek Marsh
Jan 13, 2004·Neuron·Benjamin Wolozin
Nov 30, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Cinzia EspositoAnna Maria D'ursi
Mar 27, 2007·Biochimica Et Biophysica Acta·Katsumi Matsuzaki
Oct 9, 2007·Biochimica Et Biophysica Acta·Tong-Lay LauFrances Separovic

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Citations

Jan 1, 2015·PloS One·Matilde Sublimi SaponettiAnna Maria D'Ursi
Jan 28, 2016·Colloids and Surfaces. B, Biointerfaces·Andreia CucoAmélia Gonçalves da Silva
Jan 12, 2010·Journal of Peptide Science : an Official Publication of the European Peptide Society·Giuseppe VitielloGerardino D'Errico
Jun 6, 2012·International Journal of Alzheimer's Disease·Sergio A Rosales-CorralRussel J Reiter
Mar 2, 2011·Physiology·Paul H AxelsenIan V J Murray
Jan 30, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·John M Sanderson
Apr 11, 2013·Physical Chemistry Chemical Physics : PCCP·Jean-François LabbéMichèle Auger
May 11, 2019·Scientific Reports·Amy K Smith, Dmitri K Klimov
Mar 21, 2013·ACS Chemical Neuroscience·Coralie Di ScalaJacques Fantini
Oct 23, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Giuseppe VitielloGerardino D'Errico
Nov 19, 2019·Journal of Chemical Information and Modeling·Amy K SmithDmitri K Klimov
Oct 3, 2020·ACS Chemical Neuroscience·Elias KhayatAmy K Smith

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