Interaction between arachidonic acid and cAMP signaling pathways enhances steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells

Molecular and Cellular Endocrinology
XingJia WangD M Stocco

Abstract

Previous studies have demonstrated that trophic hormone stimulation induced cyclic AMP (cAMP) formation and arachidonic acid (AA) release from phospholipids and that both these compounds were required for steroid biosynthesis and steroidogenic acute regulatory (StAR) gene expression in MA-10 mouse Leydig tumor cells. The present study further investigates the synergistic effects of the AA and cAMP interaction on steroidogenesis. To demonstrate cAMP-induced AA release, MA-10 cells were pre-loaded with 3H-AA and subsequently treated with dibutyryl cyclic AMP (dbcAMP). Stimulation with dbcAMP significantly induced AA release in MA-10 cells to a level 145.7% higher than that of controls. Lowering intracellular cAMP concentration by expressing a cAMP-phosphodiesterase significantly reduced human chorionic gonadotrophin (hCG)-induced AA release. The dbcAMP-induced AA release was inhibited significantly by the phospholipase A(2) (PLA(2)) inhibitor dexamethasone (Dex) and also by the protein kinase A (PKA) inhibitor H89, suggesting the involvement of PKA phosphorylation and/or PLA(2) activation in cAMP-induced AA release. The effect of the interaction between AA and cAMP on StAR gene expression and steroid production was also investiga...Continue Reading

References

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Citations

Jun 9, 2005·Molecular and Cellular Endocrinology·XingJia Wang, Douglas M Stocco
Feb 29, 2008·The British Journal of Nutrition·Claudia BoccaAntonella Miglietta
Jul 23, 2013·PLoS Genetics·Marianna OrlovaErwin Schurr
Apr 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Claudia E MohnValeria Rettori
Sep 2, 2008·Molecular and Cellular Endocrinology·Andrew S MidzakBarry R Zirkin
Dec 13, 2006·Experimental Neurology·Ming-Cheng Tsai, Yi-Hung Chen
Feb 1, 2005·Life Sciences·Chia-Hsien Lin, Ming-Cheng Tsai
Jul 18, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Rajani M GeorgeJeanne Wilson-Rawls
Nov 20, 2012·Molecular and Cellular Endocrinology·Mariana CookeFabiana Cornejo Maciel
Nov 9, 2016·World Journal of Stem Cells·Taylor C PeakWayne J Hellstrom
Jul 9, 2005·Endocrinology·XingJia WangDouglas M Stocco
Oct 28, 2006·Endocrinology·Haolin ChenBarry R Zirkin
Jan 18, 2003·Endocrine Research·Rocío Castilla LozanoErnesto J Podestá

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