PMID: 8596715Feb 1, 1996Paper

Interaction between capacitative Ca2+ influx and Ca2+-dependent Cl- currents in Xenopus oocytes

Pflügers Archiv : European journal of physiology
A B Parekh

Abstract

The relationship between capacitative Ca2+ influx and activation of Ca2+-dependent Cl- channels was monitored in intact Xenopus oocytes following stimulation of 5-hydroxytryptamine (5-HT) receptors, through the activity of Ca2+-dependent Cl- channels using the double-electrode voltage-clamp technique. Under the voltage-clamp conditions, t-HT evoked a rapid transient inward current followed by a slowly developing secondary inward current. The secondary current reflected depletion-activated Ca2+ entry. Hyperpolarising pulses evoked sustained Ca2+-dependent Cl- currents when applied during the transient inward current, but evoked hump-like currents which inactivated rapidly when applied during the secondary inward current. Hump currents arose from Ca2+ entering through the depletion-activated pathway. The hump currents inactivated with hyperpolarising pulses at < 5-s intervals, and recovered monoexponentially with a time constant of around 8 s. Currents in response to hyperpolarising pulses during the transient current did not inactivate, suggesting that inactivation was associated with Ca2+ entry. When Ca2+ release evoked by inositol 1,4,5-triphosphate [Ins(1,4,5)P3] was prevented by heparin injection, hyperpolarising pulses duri...Continue Reading

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