Interaction between cauliflower mosaic virus inclusion body protein and capsid protein: implications for viral assembly

Virology
A HimmelbachT Hohn

Abstract

The cauliflower mosaic virus (CaMV) inclusion body protein (pVI) is able to specifically interact with the viral capsid precursor protein (pIV). By using the yeast two-hybrid system and a blot assay, the pIV region required for the recognition of pVI was mapped to the lysine-rich domain. This region of only 48 amino acids when fused to dihydrofolate reductase (DHFR) mediated pVI and DNA binding in vitro. Competition experiments confirmed that pVI and DNA bind to the same region of pIV. Since pVI is absent from the mature virus, models are discussed in which pVI plays an accessory role in CaMV assembly, in addition to its function in transactivating translation.

Citations

Nov 20, 2002·Molecular Plant-microbe Interactions : MPMI·Yongzhong Li, Scott M Leisner
Feb 9, 2000·Journal of Virology·O Guerra-PerazaM Hemmings-Mieszczak
Oct 22, 2002·Journal of Virology·Yvan ChapdelaineDenis Leclerc
Nov 1, 2002·Molecular Plant Pathology·Muriel HaasMario Keller
Dec 1, 1996·Trends in Microbiology·Z Kiss-László, T Hohn
Dec 22, 2015·Journal of Experimental Botany·James E SchoelzScott M Leisner
Jun 21, 2011·Biochimica Et Biophysica Acta·Thomas Hohn, Franck Vazquez
Dec 6, 2005·Virus Research·Lyubov A RyabovaThomas Hohn
Jun 21, 2002·The Journal of General Virology·Aletta KarsiesDenis Leclerc
Dec 17, 1997·Biochemical and Biophysical Research Communications·V FilippovS S Gill
Sep 8, 2011·Molecular Plant·James E SchoelzRichard S Nelson
Sep 26, 2013·Current Opinion in Virology·Thomas Hohn, Helen Rothnie
Feb 22, 2008·Virology·Ho-Jong JuJeanmarie Verchot-Lubicz
Nov 22, 2014·Frontiers in Plant Science·Asako UchiyamaSondra G Lazarowitz
Jun 1, 2018·Annual Review of Phytopathology·Scott M Leisner, James E Schoelz
Jul 24, 2004·Biochemical Society Transactions·L RyabovaT Hohn
Feb 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Anders HafrénDaniel Hofius
Nov 23, 2017·Frontiers in Plant Science·James E Schoelz, Scott Leisner
Dec 16, 1998·Journal of Virology·D LeclercT Hohn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.