Interaction between cells and poly(ethylene glycol)-lipid conjugates

Colloids and Surfaces. B, Biointerfaces
Toru ItagakiHiroo Iwata

Abstract

Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15 min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1 × 10(-3) to 1 × 10(-2)molecules/nm(2), depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.

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Citations

Mar 3, 2020·Journal of Diabetes Science and Technology·Sydney C WigginsCherie L Stabler
Apr 14, 2020·Angewandte Chemie·Derek C Church, Jonathan K Pokorski
Jun 20, 2019·Frontiers in Bioengineering and Biotechnology·Shuixan Hu, Paul de Vos
Dec 14, 2018·Journal of Biological Engineering·Daniel Y LeeYoung-Wook Won
May 1, 2016·ChemNanoMat : Chemistry of Nanomaterials for Energy, Biology and More·Catarina A Custódio, João F Mano
Feb 5, 2019·Advanced Drug Delivery Reviews·Alexander U ErnstMinglin Ma
Nov 5, 2020·Bioconjugate Chemistry·Shambojit RoyAndrew P Goodwin

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